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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P17302 |
| Gene Id | 2697 |
| Gene Symbols | GJA1 |
| Protein Name / Synonyms | Connexin 43 (N-term) |
| Clonality | Polyclonal |
| Preparation | This Connexin 43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-138 amino acids from the N-terminal region of human Connexin 43. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5.
Specificity
Predicted Reactivity
Bovine, Xenopus
Other Names
GJA1; GJAL; Gap junction alpha-1 protein; Connexin-43; Gap junction 43 kDa heart protein
NCBI Accession #
NP_000156.1
Other Accession #
P16863; P18246
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
43008
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of lysates from Hela, U-87 MG, C6 cell line and mouse heart tissue lysate (from left to right), using GJA1 Antibody (N121)(Cat# 102-12784). 102-12784 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane. |
 | (LEFT)Western blot analysis of anti-GJA1 Antibody (N-term)(Cat# 102-12784) in CEM cell line lysates (35ug/lane). GJA1(arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of hGJA1-N121.Connexin (Cat# 102-12784) in mouse brain tissue lysates (35ug/lane).GJA1 (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.