Anti-Connexin 43 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P17302
Gene Id	2697
Gene Symbols	GJA1
Protein Name / Synonyms	Connexin 43 (N-term)
Clonality	Polyclonal
Preparation	This Connexin 43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-138 amino acids from the N-terminal region of human Connexin 43.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5.

Specificity

Predicted Reactivity

Bovine, Xenopus

Other Names

GJA1; GJAL; Gap junction alpha-1 protein; Connexin-43; Gap junction 43 kDa heart protein

NCBI Accession #

NP_000156.1

Other Accession #

P16863; P18246

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

43008

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of lysates from Hela, U-87 MG, C6 cell line and mouse heart tissue lysate (from left to right), using GJA1 Antibody (N121)(Cat# 102-12784). 102-12784 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
	(LEFT)Western blot analysis of anti-GJA1 Antibody (N-term)(Cat# 102-12784) in CEM cell line lysates (35ug/lane). GJA1(arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of hGJA1-N121.Connexin (Cat# 102-12784) in mouse brain tissue lysates (35ug/lane).GJA1 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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