DHRS9 Polyclonal Antibody
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9BPW9 |
| Gene Id | 10170 |
| Gene Symbols | DHRS9 |
| Protein Name / Synonyms | DHRS9, 3-alpha-HSD, 3ALPHA-HSD, RDH-E2, RDH-TBE, RDH15, RDHL, RDHTBE, RETSDR8, SDR9C4, dehydrogenase/reductase 9 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. This protein demonstrates oxidoreductase activity toward hydroxysteroids and is able to convert 3-alpha-tetrahydroprogesterone to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone in the cytoplasm, and may additionally function as a transcriptional repressor in the nucleus. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100
IHC 1:50 - 1:200
IF 1:10 - 1:100
Format
Calculated MW
18kDa/30kDa/35kDa/41kDa
Observed MW
33kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 18-319 of human DHRS9 (NP_001135743.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using DHRS9 antibody (144-06324) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using DHRS9 Antibody (144-06324) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using DHRS9 Antibody (144-06324) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using DHRS9 antibody (144-06324). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.