DNA polymerase delta antibody, 0.56 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Rabbit |
| Gene Id | 5424 |
| Protein Name / Synonyms | DNA polymerase delta |
| Concentration (lot specific) | 0.56 mg/ml |
| Recommended Applications | ICC/IF, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
CDC2 antibody, POLD antibody, POLD1 antibody, CDC2 homolog antibody, DNA-directed polymerase delta 1 antibody, DNA polymerase subunit delta p125 antibody, DNA polymerase delta catalytic subunit antibody, polymerase (DNA directed), delta 1, catalytic subunit 125kDa antibody
Specificity
Species Reactivity
Human, Rat
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
124 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 685 and 1071 of DNA pol delta cat (Uniprot ID# P28340)
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
DNA polymerase delta antibody detects POLD1 protein by Western blot analysis.
A. 30 µg Rat2 whole cell lysate/extract
5 % SDS-PAGE
DNA polymerase delta antibody (107-10354) dilution: 1:1000
A. 30 µg Rat2 whole cell lysate/extract
5 % SDS-PAGE
DNA polymerase delta antibody (107-10354) dilution: 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DNA Polymerase delta, catalytic subunit(107-10354) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
107-10354 diluted at 1:3000
A: Molt-4
7.5% SDS PAGE
107-10354 diluted at 1:3000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.