Anti-DNA polymerase delta Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Rat
Host Species	Rabbit
Gene Id	5424
Protein Name / Synonyms	DNA polymerase delta
Concentration (lot specific)	0.56 mg/ml
Recommended Applications	ICC/IF, Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

CDC2 antibody, POLD antibody, POLD1 antibody, CDC2 homolog antibody, DNA-directed polymerase delta 1 antibody, DNA polymerase subunit delta p125 antibody, DNA polymerase delta catalytic subunit antibody, polymerase (DNA directed), delta 1, catalytic subunit 125kDa antibody

Specificity

Species Reactivity

Human, Rat

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

124 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 685 and 1071 of DNA pol delta cat (Uniprot ID# P28340)

Applications

Applications Tested

ICC/IF,Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

DNA polymerase delta antibody detects POLD1 protein by Western blot analysis.
A. 30 µg Rat2 whole cell lysate/extract
5 % SDS-PAGE
DNA polymerase delta antibody (107-10354) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DNA Polymerase delta, catalytic subunit(107-10354) antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
107-10354 diluted at 1:3000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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