Name: Anti-Dupilumab ELISA Kit
SKU: EAD-AnDupi-1
Availability: InStock

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	3-5 business days
Sensitivity	15 pg/mL
Detection Range	0.015 ng/mL - 35 ng/mL
Specificity	This ELISA antibody pair detects Anti-Dupilumab. Other targets not determined.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Protein Name / Synonyms	Dupilumab
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Design Principle	Sandwich-based
Method Of Detection	Colorimetric
Research Area	Antibody Drug Development, Anti-Drug Antibody (ADA) Detection
Shipping Type	Blue ice
Storage	≤-20°C

Application Notes

Kit Components

Dupilumab Microplate
Dupilumab Antibody Standard
Biotinylated Dupilumab
HRP-Streptavidin
Wash Buffer
Assay Diluent
TMB One-Step Substrate Reagent
Stop Solution

Other Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 μL to 1 mL volumes
Adjustable 1-25 mL pipettes for reagent preparation
100 mL and 1 liter graduated cylinders
Log-log graph paper or computer and software for ELISA data analysis
Absorbent paper
Tubes to prepare standard or sample dilutions
Distilled or deionized water
Shaker

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 μL of each standard and sample to each well. Incubate for 2 hours at room temperature.
Add 100 μL prepared Biotinylated Dupilumab to each well. Incubate for 1 hour at room temperature.
Add 100 μL prepared HRP-Streptavidin solution. Incubate for 45 minutes at room temperature.
Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate for 30 minutes at room temperature.
Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Application Notes

Kit Components

Dupilumab Microplate
Dupilumab Antibody Standard
Biotinylated Dupilumab
HRP-Streptavidin
Wash Buffer
Assay Diluent
TMB One-Step Substrate Reagent
Stop Solution

Other Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 μL to 1 mL volumes
Adjustable 1-25 mL pipettes for reagent preparation
100 mL and 1 liter graduated cylinders
Log-log graph paper or computer and software for ELISA data analysis
Absorbent paper
Tubes to prepare standard or sample dilutions
Distilled or deionized water
Shaker

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 μL of each standard and sample to each well. Incubate for 2 hours at room temperature.
Add 100 μL prepared Biotinylated Dupilumab to each well. Incubate for 1 hour at room temperature.
Add 100 μL prepared HRP-Streptavidin solution. Incubate for 45 minutes at room temperature.
Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate for 30 minutes at room temperature.
Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Featured Products

Anti-Dupilumab ELISA Kit

Anti-Dupilumab ELISA Kit

Product Description

Specifications

Application Notes

Have this product in hand?

Anti-Dupilumab ELISA Kit

Product Description

Specifications

Application Notes

Have this product in hand?

Dupilumab ELISA Kit

RayPlex Dupilumab (Dupixent®) Anti-Drug Antibody Assay

RayPlex Dupilumab (Dupixent®) Drug Antibody Assay

Anti-Dupilumab ELISA Kit

Dupilumab ELISA Kit

RayPlex Dupilumab (Dupixent®) Anti-Drug Antibody Assay

RayPlex Dupilumab (Dupixent®) Drug Antibody Assay

Featured Products

Featured Products

Anti-Dupilumab ELISA Kit

Anti-Dupilumab ELISA Kit

Product Description

Specifications

Application Notes

Documents

Have this product in hand?

Reviews

Ask A Question

Anti-Dupilumab ELISA Kit

Product Description

Specifications

Related Products

Application Notes

Documents

Have this product in hand?

Reviews

Ask A Question

Related Products

Dupilumab ELISA Kit

RayPlex Dupilumab (Dupixent®) Anti-Drug Antibody Assay

RayPlex Dupilumab (Dupixent®) Drug Antibody Assay

Anti-Dupilumab ELISA Kit

Dupilumab ELISA Kit

RayPlex Dupilumab (Dupixent®) Anti-Drug Antibody Assay

RayPlex Dupilumab (Dupixent®) Drug Antibody Assay

Featured Products