Eif2ak2 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P19525 |
| Gene Id | 5610 |
| Gene Symbols | EIF2AK2 |
| Protein Name / Synonyms | EIF2AK2, EIF2AK1, PKR, PPP1R83, PRKR, eukaryotic translation initiation factor 2 alpha kinase 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
IHC 1:100 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
57kDa/62kDa
Observed MW
73kDa, 80kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human Eif2ak2 (NP_002750.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using Eif2ak2 antibody (144-05578) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.![Western blot - [KO Validated] Eif2ak2 Polyclonal Antibody (144-05578)](https://cdn.sanity.io/images/g51potcz/production/0dad6779963b970ec0a14ced8faa13502f77666b-485x350.jpg)
Western blot analysis of extracts from normal (control) and EIF2AK2 knockout (KO) HeLa cells, using EIF2AK2 antibody (144-05578) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.![Western blot - [KO Validated] Eif2ak2 Polyclonal Antibody (144-05578)](https://cdn.sanity.io/images/g51potcz/production/199f29a4abb85111d73293a97da9834d6b468779-350x350.jpg)
Immunohistochemistry of paraffin-embedded human liver using Eif2ak2 antibody (144-05578) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] Eif2ak2 Polyclonal Antibody (144-05578)](https://cdn.sanity.io/images/g51potcz/production/7e3991eb227ab503016de6eb8d3c220ed19eb931-368x550.jpg)
Immunofluorescence analysis of U2OS cells using EIF2AK2 antibody (144-05578) at dilution of 1:100. Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] Eif2ak2 Polyclonal Antibody (144-05578)](https://www.raybiotech.com/files/images/Antibodies/144/144-05578/4.jpg)
Expiration:
12 months from the date of shipment when stored properly.