ErbB2 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P04626 |
| Gene Id | 2064 |
| Protein Name / Synonyms | ERBB2, CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1, MLN19, erb-b2 receptor tyrosine kinase 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Angiogenesis, Cardiovascular Disease, Cancer, Tyrosine Kinase Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
62kDa/70kDa/97kDa/134kDa/136kDa/137kDa
Observed MW
185kDa
Immunogen
A synthetic peptide of human ErbB2
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ErbB2 antibody (144-02071) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.![Western blot - [KO Validated] ErbB2 Polyclonal Antibody (144-02071)](https://cdn.sanity.io/images/g51potcz/production/ab2bc4403cde0cb8a9b1ba4b88575c1f56e969d1-348x350.jpg)
Western blot analysis of extracts from normal (control) and ErbB2 knockout (KO) HeLa cells, using ErbB2 antibody (144-02071) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5min.![Western blot - [KO Validated] ErbB2 Polyclonal Antibody (144-02071)](https://cdn.sanity.io/images/g51potcz/production/5b9762d69416132b62b5885593cec07347f34056-350x350.jpg)
Immunohistochemistry of paraffin-embedded rat lung using ErbB2 antibody (144-02071) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] ErbB2 Polyclonal Antibody (144-02071)](https://cdn.sanity.io/images/g51potcz/production/d0039184f61f0e79aac6bbe5f3030e7d82e853fa-350x350.jpg)
Immunohistochemistry of paraffin-embedded rat ovary using ErbB2 antibody (144-02071) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] ErbB2 Polyclonal Antibody (144-02071)](https://cdn.sanity.io/images/g51potcz/production/22a1fdfdfc2348a272914d22ec46f6dfdff56681-350x350.jpg)
Immunohistochemistry of paraffin-embedded human tonsil using ErbB2 antibody (144-02071) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] ErbB2 Polyclonal Antibody (144-02071)](https://cdn.sanity.io/images/g51potcz/production/1b403ed8091e7d860940b357fb0c30cd8d4f6e72-350x350.jpg)
Immunohistochemistry of paraffin-embedded human lung cancer using ErbB2 antibody (144-02071) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] ErbB2 Polyclonal Antibody (144-02071)](https://cdn.sanity.io/images/g51potcz/production/eacc419c0a74a7826d2c4d454a790c595176ac35-350x350.jpg)
Immunohistochemistry of paraffin-embedded mouse kidney using ErbB2 antibody (144-02071) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] ErbB2 Polyclonal Antibody (144-02071)](https://www.raybiotech.com/files/images/Antibodies/144/144-02071/7.jpg)
Expiration:
12 months from the date of shipment when stored properly.