Anti-ErbB2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P04626
Gene Id	2064
Protein Name / Synonyms	ERBB2, CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1, MLN19, erb-b2 receptor tyrosine kinase 2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Angiogenesis, Cardiovascular Disease, Cancer, Tyrosine Kinase Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

62kDa/70kDa/97kDa/134kDa/136kDa/137kDa

Observed MW

185kDa

Immunogen

A synthetic peptide of human ErbB2

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ErbB2 antibody (144-02071) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] ErbB2 Polyclonal Antibody (144-02071)

Western blot analysis of extracts from normal (control) and ErbB2 knockout (KO) HeLa cells, using ErbB2 antibody (144-02071) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5min.
Western blot - [KO Validated] ErbB2 Polyclonal Antibody (144-02071)

Immunohistochemistry of paraffin-embedded rat lung using ErbB2 antibody (144-02071) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] ErbB2 Polyclonal Antibody (144-02071)

Immunohistochemistry of paraffin-embedded rat ovary using ErbB2 antibody (144-02071) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] ErbB2 Polyclonal Antibody (144-02071)

Immunohistochemistry of paraffin-embedded human tonsil using ErbB2 antibody (144-02071) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] ErbB2 Polyclonal Antibody (144-02071)

Immunohistochemistry of paraffin-embedded human lung cancer using ErbB2 antibody (144-02071) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] ErbB2 Polyclonal Antibody (144-02071)

Immunohistochemistry of paraffin-embedded mouse kidney using ErbB2 antibody (144-02071) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] ErbB2 Polyclonal Antibody (144-02071)

Expiration:

12 months from the date of shipment when stored properly.

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