Synonyms
CKN1 antibody, CSA antibody, ERCC8 antibody, Cockayne syndrome WD-repeat protein CSA antibody, DNA excision repair protein ERCC-8 antibody, cockayne syndrome WD repeat protein CSA antibody, excision repair cross-complementing rodent repair deficiency, complementation group 8 antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
44 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 194 of CSA (Uniprot ID# Q13216)
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

ERCC8 antibody detects ERCC8 protein by Western blot analysis.
A. 30 µg C8D30 whole cell lysate/extract
10 % SDS-PAGE
ERCC8 antibody (107-10184) dilution: 1:1000

ERCC8 antibody detects ERCC8 protein by Western blot analysis.
A. 30 µg Rat2 whole cell lysate/extract
10 % SDS-PAGE
ERCC8 antibody (107-10184) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ERCC8(107-10184) antibody at 1:200 dilution.

Sample (30µg whole cell lysate)
A:MOLT4
10% SDS PAGE
107-10184 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.