Anti-ERCC8 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Gene Id	1161
Protein Name / Synonyms	ERCC8
Concentration (lot specific)	5.39 mg/ml
Recommended Applications	ICC/IF, Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

CKN1 antibody, CSA antibody, ERCC8 antibody, Cockayne syndrome WD-repeat protein CSA antibody, DNA excision repair protein ERCC-8 antibody, cockayne syndrome WD repeat protein CSA antibody, excision repair cross-complementing rodent repair deficiency, complementation group 8 antibody

Specificity

Species Reactivity

Human, Rat, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

44 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 194 of CSA (Uniprot ID# Q13216)

Applications

Applications Tested

ICC/IF,Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

ERCC8 antibody detects ERCC8 protein by Western blot analysis.
A. 30 µg C8D30 whole cell lysate/extract
10 % SDS-PAGE
ERCC8 antibody (107-10184) dilution: 1:1000

ERCC8 antibody detects ERCC8 protein by Western blot analysis.
A. 30 µg Rat2 whole cell lysate/extract
10 % SDS-PAGE
ERCC8 antibody (107-10184) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ERCC8(107-10184) antibody at 1:200 dilution.

Sample (30µg whole cell lysate)
A:MOLT4
10% SDS PAGE
107-10184 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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