GNRH1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P01148 |
| Gene Id | 2796 |
| Gene Symbols | GNRH1 |
| Protein Name / Synonyms | GNRH1, GNRH, GRH, LHRH, LNRH, progonadoliberin-1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a preproprotein that is proteolytically processed to generate a peptide that is a member of the gonadotropin-releasing hormone (GnRH) family of peptides. Alternative splicing results in multiple transcript variants, at least one of which is secreted and then cleaved to generate gonadoliberin-1 and GnRH-associated peptide 1. Gonadoliberin-1 stimulates the release of luteinizing and follicle stimulating hormones, which are important for reproduction. Mutations in this gene are associated with hypogonadotropic hypogonadism.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
10kDa
Observed MW
12kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 24-92 of human GNRH1 (NP_001076580.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using GNRH1 antibody (144-05625) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human prostate using GNRH1 antibody (144-05625) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using GNRH1 antibody (144-05625) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.