Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q16666
Gene Id	3428
Gene Symbols	IFI16
Protein Name / Synonyms	IFI16, IFNGIP1, PYHIN2, gamma-interferon-inducible protein 16
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Inflammation, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Background

This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

75kDa/82kDa/88kDa

Observed MW

88kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human IFI16 (NP_005522.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using IFI16 antibody (144-60546) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - IFI16 antibody (144-60546)

Immunohistochemistry of paraffin-embedded rat intestine using IFI16 Antibody (144-60546) at dilution of 1:100 (40x lens).
Immunohistochemistry - IFI16 Antibody (144-60546)

Immunohistochemistry of paraffin-embedded rat lung using IFI16 Antibody (144-60546) at dilution of 1:100 (40x lens).
Immunohistochemistry - IFI16 Antibody (144-60546)

Immunohistochemistry of paraffin-embedded rat liver using IFI16 Antibody (144-60546) at dilution of 1:100 (40x lens).
Immunohistochemistry - IFI16 Antibody (144-60546)

Immunohistochemistry of paraffin-embedded rat kidney using IFI16 Antibody (144-60546) at dilution of 1:100 (40x lens).
Immunohistochemistry - IFI16 Antibody (144-60546)

Immunohistochemistry of paraffin-embedded human kidney cancer using IFI16 Antibody (144-60546) at dilution of 1:100 (40x lens).
Immunohistochemistry - IFI16 Antibody (144-60546)

Immunohistochemistry of paraffin-embedded mouse lung using IFI16 Antibody (144-60546) at dilution of 1:100 (40x lens).
Immunohistochemistry - IFI16 Antibody (144-60546)

Immunohistochemistry of paraffin-embedded mouse liver using IFI16 Antibody (144-60546) at dilution of 1:100 (40x lens).
Immunohistochemistry - IFI16 Antibody (144-60546)

Immunohistochemistry of paraffin-embedded mouse kidney using IFI16 Antibody (144-60546) at dilution of 1:100 (40x lens).
Immunohistochemistry - IFI16 Antibody (144-60546)

Expiration:

12 months from the date of shipment when stored properly.