IRF8 Polyclonal Antibody
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q02556 |
| Gene Id | 3394 |
| Gene Symbols | IRF8 |
| Protein Name / Synonyms | IRF8, H-ICSBP, ICSBP, ICSBP1, IMD32A, IMD32B, IRF-8, interferon regulatory factor 8 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
48kDa
Observed MW
54kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 137-426 of human IRF8 (NP_002154.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of THP-1 cells, using IRF8 antibody (144-60440) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunofluorescence analysis of A549 cells using IRF8 antibody (144-60440).
Expiration:
12 months from the date of shipment when stored properly.