KDM1A Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O60341 |
| Gene Id | 23028 |
| Gene Symbols | KDM1A |
| Protein Name / Synonyms | KDM1A, AOF2, BHC110, CPRF, KDM1, LSD1, lysine demethylase 1A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IP 1:50 - 1:100
IHC 1:50 - 1:100
IF 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
92kDa/95kDa
Observed MW
96-117kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 130-380 of human KDM1A (NP_055828.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using KDM1A antibody (144-01156) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.![Western blot - [KO Validated] KDM1A Polyclonal Antibody (144-01156)](https://cdn.sanity.io/images/g51potcz/production/572fa0a8ab2b6586500a977ef9c04ca48c0678de-245x350.jpg)
Western blot analysis of extracts from normal (control) and KDM1A knockout (KO) HeLa cells, using KDM1A antibody (144-01156) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.![Western blot - [KO Validated] KDM1A Polyclonal Antibody (144-01156)](https://cdn.sanity.io/images/g51potcz/production/75581b15392724a49ce8b37b2494ebff3234cb52-468x350.jpg)
Immunofluorescence analysis of A549 cells using KDM1A antibody (144-01156).![Immunofluorescence - [KO Validated] KDM1A Polyclonal Antibody (144-01156)](https://cdn.sanity.io/images/g51potcz/production/1d76f92af953955277d7acbfe7bb1a3a0e753e3a-266x350.jpg)
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug KDM1A antibody (144-01156). Western blot was performed from the immunoprecipitate using KDM1A antibody (144-01156) at a dilition of 1:500.![Immunoprecipitation - [KO Validated] KDM1A Polyclonal Antibody (144-01156)](https://www.raybiotech.com/files/images/Antibodies/144/144-01156/4.jpg)
Expiration:
12 months from the date of shipment when stored properly.
