L3MBTL3 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q96JM7 |
| Gene Id | 84456 |
| Gene Symbols | L3MBTL3 |
| Protein Name / Synonyms | L3MBTL3, MBT-1, MBT1, l(3)mbt-like 3 (Drosophila) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:100
IP 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:200
Format
Calculated MW
85kDa/88kDa
Observed MW
110kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human L3MBTL3 (NP_001007103.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using L3MBTL3 antibody (144-07289) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
Immunofluorescence analysis of MCF-7 cells using L3MBTL3 antibody (144-07289). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug L3MBTL3 antibody (144-07289). Western blot was performed from the immunoprecipitate using L3MBTL3 antibody (144-07289) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.