MNAT1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P51948 |
| Gene Id | 4331 |
| Gene Symbols | MNAT1 |
| Protein Name / Synonyms | MNAT1, CAP35, MAT1, RNF66, TFB3, CDK-activating kinase assembly factor MAT1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IP |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene, along with cyclin H and CDK7, forms the CDK-activating kinase (CAK) enzymatic complex. This complex activates several cyclin-associated kinases and can also associate with TFIIH to activate transcription by RNA polymerase II. Two transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IP 1:50 - 1:200
IP 1:50 - 1:200
Format
Calculated MW
31kDa/35kDa
Observed MW
36kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human MNAT1 (NP_002422.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using MNAT1 antibody (144-01918) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug MNAT1 antibody (144-01918). Western blot was performed from the immunoprecipitate using MNAT1 antibody (144-01918) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.
