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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P16070 |
| Gene Id | 960 |
| Gene Symbols | CD44 |
| Protein Name / Synonyms | CD44, CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, CD44 antigen |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cardiovascular Disease, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
3kDa/15kDa/32-53kDa/73-81kDa
Observed MW
82kDa
Immunogen
Recombinant protein of human CD44
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of mouse spleen, using CD44 Antibody (144-012410) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.![Western blot - [KO Validated] CD44 Polyclonal Antibody (144-012410)](https://cdn.sanity.io/images/g51potcz/production/42f1e3833427f5832a72dcd1b1d55c541328bcf4-200x350.jpg)
Western blot analysis of extracts from normal (control) and CD44 knockout (KO) HeLa cells, using CD44 antibody (144-012410) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.![Western blot - [KO Validated] CD44 Polyclonal Antibody (144-012410)](https://cdn.sanity.io/images/g51potcz/production/0d234f1377fecba03a89a0741f601626f78bd466-350x350.jpg)
Immunohistochemistry of paraffin-embedded rat bronchus using CD44 antibody (144-012410) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] CD44 Polyclonal Antibody (144-012410)](https://cdn.sanity.io/images/g51potcz/production/4ed2623b120e04271b0490b2bfc281816e1a21f1-440x550.jpg)
Immunofluorescence analysis of HeLa cells using CD44 antibody (144-012410) at dilution of 1:100. Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] CD44 Polyclonal Antibody (144-012410)](https://www.raybiotech.com/files/images/Antibodies/144/144-12410/4.jpg)
Expiration:
12 months from the date of shipment when stored properly.
