NDUFS3 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O75489 |
| Gene Id | 4722 |
| Gene Symbols | NDUFS3 |
| Protein Name / Synonyms | NDUFS3, CI-30, NADH:ubiquinone oxidoreductase core subunit S3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
14kDa/30kDa
Observed MW
30kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 37-264 of human NDUFS3 (NP_004542.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using NDUFS3 antibody (144-08013) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.![Western blot - [KO Validated] NDUFS3 Polyclonal Antibody (144-08013)](https://cdn.sanity.io/images/g51potcz/production/91b25c4a231d6c9755088e71c2ac0561b9484b27-383x350.jpg)
Western blot analysis of extracts from normal (control) and NDUFS3 knockout (KO) 293T cells, using NDUFS3 antibody (144-08013) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.![Western blot - [KO Validated] NDUFS3 Polyclonal Antibody (144-08013)](https://cdn.sanity.io/images/g51potcz/production/45480dd1b9f97c01ae3b3193e128ee93671af3be-350x350.jpg)
Immunohistochemistry of paraffin-embedded human liver injury using NDUFS3 antibody (144-08013) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] NDUFS3 Polyclonal Antibody (144-08013)](https://cdn.sanity.io/images/g51potcz/production/f518dcf310c3d90cbb0971f13048cbf9bc073939-350x350.jpg)
Immunohistochemistry of paraffin-embedded rat brain using NDUFS3 antibody (144-08013) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] NDUFS3 Polyclonal Antibody (144-08013)](https://www.raybiotech.com/files/images/Antibodies/144/144-08013/4.jpg)
Expiration:
12 months from the date of shipment when stored properly.