Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q15233
Gene Id	4841
Gene Symbols	NONO
Protein Name / Synonyms	NONO, MRXS34, NMT55, NRB54, P54, P54NRB, PPP1R114, non-POU domain containing, octamer-binding
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Background

This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

43kDa/54kDa

Observed MW

58kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NONO (NP_001138881.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using Nono antibody (144-05282) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - Nono antibody (144-05282)

Immunohistochemistry of paraffin-embedded rat liver using Nono Antibody (144-05282) at dilution of 1:100 (40x lens).
Immunohistochemistry - Nono Antibody (144-05282)

Immunohistochemistry of paraffin-embedded rat brain using Nono Antibody (144-05282) at dilution of 1:100 (40x lens).
Immunohistochemistry - Nono Antibody (144-05282)

Immunohistochemistry of paraffin-embedded human colon using Nono Antibody (144-05282) at dilution of 1:100 (40x lens).
Immunohistochemistry - Nono Antibody (144-05282)

Immunohistochemistry of paraffin-embedded human esophagus using Nono Antibody (144-05282) at dilution of 1:100 (40x lens).
Immunohistochemistry - Nono Antibody (144-05282)

Immunohistochemistry of paraffin-embedded human gastric cancer using Nono Antibody (144-05282) at dilution of 1:100 (40x lens).
Immunohistochemistry - Nono Antibody (144-05282)

Immunohistochemistry of paraffin-embedded mouse lung using Nono Antibody (144-05282) at dilution of 1:100 (40x lens).
Immunohistochemistry - Nono Antibody (144-05282)

Immunohistochemistry of paraffin-embedded mouse brain using Nono Antibody (144-05282) at dilution of 1:100 (40x lens).
Immunohistochemistry - Nono Antibody (144-05282)

Immunohistochemistry of paraffin-embedded mouse heart using Nono Antibody (144-05282) at dilution of 1:100 (40x lens).
Immunohistochemistry - Nono Antibody (144-05282)

Immunofluorescence analysis of U2OS cells using Nono antibody (144-05282).
Immunofluorescence - Nono antibody (144-05282)

Expiration:

12 months from the date of shipment when stored properly.