Phospho-Bcl2 -S70 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Rabbit |
| Accession Number | P10415 |
| Gene Id | 596 |
| Gene Symbols | BCL2 |
| Protein Name / Synonyms | BCL2, Bcl-2, PPP1R50, apoptosis regulator Bcl-2 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Research Area | Apoptosis, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
22kDa/26kDa
Observed MW
26kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding S70 of human Bcl2
Modification
Phospho S70
Storage
-20°C
Images
Western blot analysis of extracts of Jurkat and 293 cells, using Phospho-Bcl2 -S70 antibody (144-50575) at 1:1000 dilution. Jurkat cells were treated by serum-starvation overnight. 293 cells were treated by UV for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Immunofluorescence analysis of HeLa cells using BCL2 antibody (144-50575) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.
