Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P40763
Gene Id	6774
Gene Symbols	STAT3
Protein Name / Synonyms	STAT3, ADMIO, ADMIO1, APRF, HIES, signal transducer and activator of transcription 3
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.05% proclin300, 50% glycerol, pH 7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Angiogenesis, Cancer, Inflammation, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

83kDa/87kDa/88kDa

Observed MW

105kDa

Immunogen

A synthetic phosphorylated peptide around Y705 of human Stat3 (NP_644805.1).

Modification

Phospho Y705

Storage

-20°C

Images

Western blot analysis of extracts of HeLa cells, using Phospho-Stat3-Y705 antibody (144-50070) at 1:2000 dilution or STAT3 antibody (144-01192). HeLa cells were treated by IFN-? (100ng/ml) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - Phospho-Stat3-Y705 pAb (144-50070)

Immunohistochemistry of paraffin-embedded rat lung using Phospho-Stat3-Y705 antibody (144-50070) at dilution of 1:100 (40x lens).
Immunohistochemistry - Phospho-Stat3-Y705 pAb (144-50070)

Immunohistochemistry of paraffin-embedded rat liver using Phospho-Stat3-Y705 antibody (144-50070) at dilution of 1:100 (40x lens).
Immunohistochemistry - Phospho-Stat3-Y705 pAb (144-50070)

Immunohistochemistry of paraffin-embedded rat testis using Phospho-Stat3-Y705 antibody (144-50070) at dilution of 1:100 (40x lens).
Immunohistochemistry - Phospho-Stat3-Y705 pAb (144-50070)

Immunohistochemistry of paraffin-embedded rat brain using Phospho-Stat3-Y705 antibody (144-50070) at dilution of 1:100 (40x lens).
Immunohistochemistry - Phospho-Stat3-Y705 pAb (144-50070)

Immunohistochemistry of paraffin-embedded rat spleen using Phospho-Stat3-Y705 antibody (144-50070) at dilution of 1:100 (40x lens).
Immunohistochemistry - Phospho-Stat3-Y705 pAb (144-50070)

Immunohistochemistry of paraffin-embedded rat heart using Phospho-Stat3-Y705 antibody (144-50070) at dilution of 1:100 (40x lens).
Immunohistochemistry - Phospho-Stat3-Y705 pAb (144-50070)

Immunohistochemistry of paraffin-embedded human tonsil using Phospho-Stat3-Y705 antibody (144-50070) at dilution of 1:100 (40x lens).
Immunohistochemistry - Phospho-Stat3-Y705 pAb (144-50070)

Immunohistochemistry of paraffin-embedded human lung using Phospho-Stat3-Y705 antibody (144-50070) at dilution of 1:100 (40x lens).
Immunohistochemistry - Phospho-Stat3-Y705 pAb (144-50070)

Expiration:

12 months from the date of shipment when stored properly.