Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O00444
Gene Id	10733
Gene Symbols	PLK4
Protein Name / Synonyms	PLK4, MCCRP2, SAK, STK18, polo like kinase 4
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Background

This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. Three alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

104kDa/105kDa/108kDa

Observed MW

115kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 641-970 of human PLK4 (NP_055079.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PLK4 antibody (144-09863) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Western blot - PLK4 antibody (144-09863)

Immunohistochemistry of paraffin-embedded rat lung using PLK4 antibody (144-09863) at dilution of 1:100 (40x lens).
Immunohistochemistry - PLK4 antibody (144-09863)

Immunohistochemistry of paraffin-embedded rat brain using PLK4 antibody (144-09863) at dilution of 1:100 (40x lens).
Immunohistochemistry - PLK4 antibody (144-09863)

Immunohistochemistry of paraffin-embedded rat kidney using PLK4 antibody (144-09863) at dilution of 1:100 (40x lens).
Immunohistochemistry - PLK4 antibody (144-09863)

Immunohistochemistry of paraffin-embedded human lung using PLK4 antibody (144-09863) at dilution of 1:100 (20x lens).
Immunohistochemistry - PLK4 antibody (144-09863)

Immunohistochemistry of paraffin-embedded human prostate using PLK4 antibody (144-09863) at dilution of 1:100 (40x lens).
Immunohistochemistry - PLK4 antibody (144-09863)

Immunohistochemistry of paraffin-embedded human esophagus using PLK4 antibody (144-09863) at dilution of 1:100 (40x lens).
Immunohistochemistry - PLK4 antibody (144-09863)

Immunohistochemistry of paraffin-embedded human stomach using PLK4 antibody (144-09863) at dilution of 1:100 (40x lens).
Immunohistochemistry - PLK4 antibody (144-09863)

Immunohistochemistry of paraffin-embedded mouse liver using PLK4 antibody (144-09863) at dilution of 1:100 (40x lens).
Immunohistochemistry - PLK4 antibody (144-09863)

Expiration:

12 months from the date of shipment when stored properly.