POLB Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P06746 |
| Gene Id | 5423 |
| Gene Symbols | POLB |
| Protein Name / Synonyms | POLB, DNA polymerase beta |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date.
Dilutions
WB 1:200 - 1:1000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
38kDa
Observed MW
37kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human POLB (NP_002681.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using POLB antibody (144-01681) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.![Western blot - [KO Validated] POLB Polyclonal Antibody (144-01681)](https://cdn.sanity.io/images/g51potcz/production/cd5dfc8204270c3e10070df60e552002057578d9-294x350.jpg)
Western blot analysis of extracts from normal (control) and POLB knockout (KO) HeLa cells, using POLB antibody (144-01681) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.![Western blot - [KO Validated] POLB Polyclonal Antibody (144-01681)](https://cdn.sanity.io/images/g51potcz/production/bb8a03406b9fb5155eb01f954286474146b1ec31-366x550.jpg)
Immunofluorescence analysis of U2OS cells using POLB antibody (144-01681). Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] POLB Polyclonal Antibody (144-01681)](https://www.raybiotech.com/files/images/Antibodies/144/144-01681/3.jpg)
Expiration:
12 months from the date of shipment when stored properly.