POLR2D Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | O15514 |
| Gene Id | 5433 |
| Gene Symbols | POLR2D |
| Protein Name / Synonyms | POLR2D, HSRBP4, HSRPB4, RBP4, RPB16, RNA polymerase II subunit D |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene.
Dilutions
WB 1:500 - 1:2000
IF 1:10 - 1:100
IP 1:50 - 1:200
IF 1:10 - 1:100
IP 1:50 - 1:200
Format
Calculated MW
16kDa
Observed MW
17kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human POLR2D (NP_004796.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using POLR2D antibody (144-01859) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of A549 cells using POLR2D antibody (144-01859).
Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug POLR2D antibody (144-01859). Western blot was performed from the immunoprecipitate using POLR2D antibody (144-01859) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.