[KO Validated] PPP1CC Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P36873 |
| Gene Id | 5501 |
| Gene Symbols | PPP1CC |
| Protein Name / Synonyms | PPP1CC, PP-1G, PP1C, PPP1G, protein phosphatase 1 catalytic subunit gamma |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene belongs to the protein phosphatase family, PP1 subfamily. PP1 is an ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. It is expressed in mammalian cells as three closely related isoforms, alpha, beta/delta and gamma, which have distinct localization patterns. This gene encodes the gamma isozyme. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:200 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
36kDa/38kDa
Observed MW
38kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human PPP1CC (NP_002701.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PPP1CC antibody (144-04035) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.![Western blot - [KO Validated] PPP1CC Polyclonal Antibody (144-04035)](https://cdn.sanity.io/images/g51potcz/production/6b6f574754070b8e030ced443cbd4f67c4f60216-587x350.jpg)
Western blot analysis of extracts from normal (control) and PPP1CC knockout (KO) HeLa cells, using PPP1CC antibody (144-04035) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.![Western blot - [KO Validated] PPP1CC Polyclonal Antibody (144-04035)](https://cdn.sanity.io/images/g51potcz/production/f6749fedb779b8b9e0a7bd60e5ad4965ca8e7620-350x350.jpg)
Immunohistochemistry of paraffin-embedded human gastric cancer using PPP1CC antibody (144-04035) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] PPP1CC Polyclonal Antibody (144-04035)](https://cdn.sanity.io/images/g51potcz/production/0b60cab6dd03c292ff93727e3fa082f88ad0c0eb-350x350.jpg)
Immunohistochemistry of paraffin-embedded rat brain using PPP1CC antibody (144-04035) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] PPP1CC Polyclonal Antibody (144-04035)](https://cdn.sanity.io/images/g51potcz/production/f7af8779255a34ef8fe17c7ced78ccb00f20d54d-350x350.jpg)
Immunohistochemistry of paraffin-embedded human stomach using PPP1CC antibody (144-04035) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] PPP1CC Polyclonal Antibody (144-04035)](https://www.raybiotech.com/files/images/Antibodies/144/144-04035/5.jpg)
Expiration:
12 months from the date of shipment when stored properly.