RAB27A Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P51159 |
| Gene Id | 5873 |
| Gene Symbols | RAB27A |
| Protein Name / Synonyms | RAB27A, GS2, HsT18676, RAB27, RAM, ras-related protein Rab-27A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100
IHC 1:50 - 1:200
IF 1:10 - 1:100
Format
Calculated MW
24kDa
Observed MW
25kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human RAB27A (NP_899057.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of U-937 cells, using RAB27A antibody (144-01934) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.![Western blot - [KO Validated] RAB27A Polyclonal Antibody (144-01934)](https://cdn.sanity.io/images/g51potcz/production/fbda65665df767171a3700e50f3e3fe82263603b-194x350.jpg)
Western blot analysis of extracts from normal (control) and RAB27A knockout (KO) HeLa cells, using RAB27A antibody (144-01934) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.![Western blot - [KO Validated] RAB27A Polyclonal Antibody (144-01934)](https://cdn.sanity.io/images/g51potcz/production/44c293df6cbac1bb7801fcace673e5b327cbf858-350x350.jpg)
Immunohistochemistry of paraffin-embedded human adenomyosis using RAB27A Antibody (144-01934) at dilution of 1:200 (40x lens).![Immunohistochemistry - [KO Validated] RAB27A Polyclonal Antibody (144-01934)](https://cdn.sanity.io/images/g51potcz/production/39ca91b126e444e3b06e335e4e5944a7f01e1fdb-350x350.jpg)
Immunohistochemistry of paraffin-embedded human liver injury using RAB27A Antibody (144-01934) at dilution of 1:200 (40x lens).![Immunohistochemistry - [KO Validated] RAB27A Polyclonal Antibody (144-01934)](https://cdn.sanity.io/images/g51potcz/production/fc73825761bee871a55edb9f8e1068840c925101-467x350.jpg)
Immunohistochemistry of paraffin-embedded human liver injury using RAB27A Antibody (144-01934) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] RAB27A Polyclonal Antibody (144-01934)](https://cdn.sanity.io/images/g51potcz/production/a0861f1753e5a82a8005ce606231c699176a6eec-467x350.jpg)
Immunohistochemistry of paraffin-embedded human adenomyosis using RAB27A Antibody (144-01934) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] RAB27A Polyclonal Antibody (144-01934)](https://www.raybiotech.com/files/images/Antibodies/144/144-01934/6.jpg)
Immunofluorescence analysis of MCF7 cells using RAB27A antibody (144-01934). Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] RAB27A Polyclonal Antibody (144-01934)](https://www.raybiotech.com/files/images/Antibodies/144/144-01934/7.jpg)
Expiration:
12 months from the date of shipment when stored properly.