Anti-RAB27A Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P51159
Gene Id	5873
Gene Symbols	RAB27A
Protein Name / Synonyms	RAB27A, GS2, HsT18676, RAB27, RAM, ras-related protein Rab-27A
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100

Format

Calculated MW

24kDa

Observed MW

25kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human RAB27A (NP_899057.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of U-937 cells, using RAB27A antibody (144-01934) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Western blot - [KO Validated] RAB27A Polyclonal Antibody (144-01934)

Western blot analysis of extracts from normal (control) and RAB27A knockout (KO) HeLa cells, using RAB27A antibody (144-01934) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] RAB27A Polyclonal Antibody (144-01934)

Immunohistochemistry of paraffin-embedded human adenomyosis using RAB27A Antibody (144-01934) at dilution of 1:200 (40x lens).
Immunohistochemistry - [KO Validated] RAB27A Polyclonal Antibody (144-01934)

Immunohistochemistry of paraffin-embedded human liver injury using RAB27A Antibody (144-01934) at dilution of 1:200 (40x lens).
Immunohistochemistry - [KO Validated] RAB27A Polyclonal Antibody (144-01934)

Immunohistochemistry of paraffin-embedded human liver injury using RAB27A Antibody (144-01934) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] RAB27A Polyclonal Antibody (144-01934)

Immunohistochemistry of paraffin-embedded human adenomyosis using RAB27A Antibody (144-01934) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] RAB27A Polyclonal Antibody (144-01934)

Immunofluorescence analysis of MCF7 cells using RAB27A antibody (144-01934). Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] RAB27A Polyclonal Antibody (144-01934)

Expiration:

12 months from the date of shipment when stored properly.

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