RARA Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P10276 |
| Gene Id | 5914 |
| Gene Symbols | RARA |
| Protein Name / Synonyms | RARA, NR1B1, RAR, retinoic acid receptor alpha |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IHC 1:50 - 1:100
IF 1:50 - 1:100
Format
Calculated MW
39kDa/50kDa
Observed MW
51kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-457 of human RARA (NP_001019980.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using RARA antibody (144-00370) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using RARA antibody (144-00370) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using RARA antibody (144-00370) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using RARA antibody (144-00370) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RARA antibody (144-00370) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse cerebellum using RARA antibody (144-00370) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.