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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P35251 |
| Gene Id | 5981 |
| Gene Symbols | RFC1 |
| Protein Name / Synonyms | RFC1, A1, MHCBFB, PO-GA, RECC1, RFC, RFC140, replication factor C subunit 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
128kDa
Observed MW
130kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 400-700 of human RFC1 (NP_001191676.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of MCF-7 cells, using RFC1 antibody (144-01625) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of MCF-7 cells using RFC1 antibody (144-01625). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of A549 cells using RFC1 antibody (144-01625).
Expiration:
12 months from the date of shipment when stored properly.
