SERPINA3 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P01011 |
| Gene Id | 12 |
| Gene Symbols | SERPINA3 |
| Protein Name / Synonyms | SERPINA3, AACT, ACT, GIG24, GIG25, alpha-1-antichymotrypsin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variations in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200
IHC 1:50 - 1:100
IF 1:50 - 1:200
Format
Calculated MW
10kDa/24kDa/47kDa
Observed MW
50kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 24-423 of human SERPINA3 (NP_001076.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SERPINA3 antibody (144-01021) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using SERPINA3 antibody (144-01021) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using SERPINA3 antibody (144-01021) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using SERPINA3 antibody (144-01021). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.
