SGK1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O00141 |
| Gene Id | 6446 |
| Gene Symbols | SGK1 |
| Protein Name / Synonyms | SGK1, SGK, serine/threonine-protein kinase Sgk1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
47kDa/48kDa/50kDa/52kDa/59kDa
Observed MW
60kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 172-431 of human SGK1 (NP_005618.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SGK1 antibody (144-01025) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Western blot analysis of extracts of various cell lines, using SGK1 antibody (144-01025) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 300s.
Immunohistochemistry of paraffin-embedded human esophagus using SGK1 antibody (144-01025) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using SGK1 antibody (144-01025). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.