SMARCC1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q92922 |
| Gene Id | 6599 |
| Gene Symbols | SMARCC1 |
| Protein Name / Synonyms | SMARCC1, BAF155, CRACC1, Rsc8, SRG3, SWI3, SWI/SNF complex subunit SMARCC1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:200
IHC 1:50 - 1:200
IP 1:50 - 1:200
Format
Calculated MW
122kDa
Observed MW
155kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 80-320 of human SMARCC1 (NP_003065.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SMARCC1 antibody (144-06128) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 120s.![Western blot - [KO Validated] SMARCC1 Polyclonal Antibody (144-06128)](https://cdn.sanity.io/images/g51potcz/production/500134f7797f8000cc6281f461798baa52b0188d-318x350.jpg)
Western blot analysis of extracts from normal (control) and SMARCC1 knockout (KO) HeLa cells, using SMARCC1 antibody (144-06128) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.![Western blot - [KO Validated] SMARCC1 Polyclonal Antibody (144-06128)](https://cdn.sanity.io/images/g51potcz/production/49462ad2059c7c43569d366f0d2da53c1cc3ccbc-350x350.jpg)
Immunohistochemistry of paraffin-embedded human kidney cancer using SMARCC1 antibody (144-06128) at dilution of 1:200 (40x lens).![Immunohistochemistry - [KO Validated] SMARCC1 Polyclonal Antibody (144-06128)](https://cdn.sanity.io/images/g51potcz/production/c8d96af1e64bcb4555678fef1b1b664862be18ff-334x350.jpg)
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug SMARCC1 antibody (144-06128). Western blot was performed from the immunoprecipitate using SMARCC1 antibody (144-06128) at a dilition of 1:1000.![Immunoprecipitation - [KO Validated] SMARCC1 Polyclonal Antibody (144-06128)](https://www.raybiotech.com/files/images/Antibodies/144/144-06128/4.jpg)
Expiration:
12 months from the date of shipment when stored properly.