[KO Validated] SMARCE1 Polyclonal Antibody
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q969G3 |
| Gene Id | 6605 |
| Gene Symbols | SMARCE1 |
| Protein Name / Synonyms | SMARCE1, BAF57, CSS5, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50
Format
Calculated MW
34kDa/37kDa/39kDa/41kDa/42kDa/46kDa
Observed MW
55kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-411 of human SMARCE1 (NP_003070.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SMARCE1 antibody (144-602973) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.![Western blot - [KO Validated] SMARCE1 Polyclonal Antibody (144-602973)](https://cdn.sanity.io/images/g51potcz/production/cb200023239fcd9f23c304bc562f3bba1f4b50b2-215x350.jpg)
Western blot analysis of extracts from normal (control) and SMARCE1 knockout (KO) HeLa cells, using SMARCE1 antibody (144-602973) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.![Western blot - [KO Validated] SMARCE1 Polyclonal Antibody (144-602973)](https://cdn.sanity.io/images/g51potcz/production/18711c9a17897f3d76e3b730487d2e4a7b8aecc0-468x350.jpg)
Immunofluorescence analysis of HeLa cells using SMARCE1 antibody (144-602973).![Immunofluorescence - [KO Validated] SMARCE1 Polyclonal Antibody (144-602973)](https://cdn.sanity.io/images/g51potcz/production/03fb7968e894170dcf7e2d90b57d9dcef560ae5d-350x350.jpg)
Immunoprecipitation analysis of 150ug extracts of Jurkat cells using 3ug SMARCE1 antibody (144-602973). Western blot was performed from the immunoprecipitate using SMARCE1 antibody (144-602973) at a dilition of 1:1000.![Immunoprecipitation - [KO Validated] SMARCE1 Polyclonal Antibody (144-602973)](https://www.raybiotech.com/files/images/Antibodies/144/144-60299/4.jpg)
Expiration:
12 months from the date of shipment when stored properly.