SUZ12 Polyclonal Antibody
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q15022 |
| Gene Id | 23512 |
| Gene Symbols | SUZ12 |
| Protein Name / Synonyms | SUZ12, CHET9, JJAZ1, polycomb protein SUZ12 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IP |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:100
IHC 1:50 - 1:200
IP 1:50 - 1:100
Format
Calculated MW
83kDa
Observed MW
83kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 470-739 of human SUZ12 (NP_056170.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SUZ12 Antibody (144-07786) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.![Western blot - [KO Validated] SUZ12 Polyclonal Antibody (144-07786)](https://cdn.sanity.io/images/g51potcz/production/f17d5796a38b76c7c627386643b0b6659f2e1af5-489x350.jpg)
Western blot analysis of extracts from normal (control) and SUZ12 knockout (KO) HeLa cells, using SUZ12 antibody (144-07786) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.![Western blot - [KO Validated] SUZ12 Polyclonal Antibody (144-07786)](https://cdn.sanity.io/images/g51potcz/production/ab9a3741ca6172db700831d54a8be01195bacbc7-350x350.jpg)
Immunohistochemistry of paraffin-embedded rat lung using SUZ12 antibody (144-07786) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] SUZ12 Polyclonal Antibody (144-07786)](https://cdn.sanity.io/images/g51potcz/production/976fa5609cf46dfae6172f924d9e15556b81a876-350x350.jpg)
Immunohistochemistry of paraffin-embedded rat brain using SUZ12 antibody (144-07786) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] SUZ12 Polyclonal Antibody (144-07786)](https://cdn.sanity.io/images/g51potcz/production/e3e4b475ebef45cd98e41162f83d1be67cfb0d56-350x350.jpg)
Immunohistochemistry of paraffin-embedded rat heart using SUZ12 antibody (144-07786) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] SUZ12 Polyclonal Antibody (144-07786)](https://cdn.sanity.io/images/g51potcz/production/ae52b62070a25cb9d041b09200f9d799ea1318a3-350x350.jpg)
Immunohistochemistry of paraffin-embedded human breast cancer using SUZ12 antibody (144-07786) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] SUZ12 Polyclonal Antibody (144-07786)](https://cdn.sanity.io/images/g51potcz/production/5335b9a5fddac783115638b56d79ac3901176c71-350x350.jpg)
Immunohistochemistry of paraffin-embedded human gastric cancer using SUZ12 antibody (144-07786) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] SUZ12 Polyclonal Antibody (144-07786)](https://cdn.sanity.io/images/g51potcz/production/9176cb37f869ab9e3098a85a8de8cd7e2803aec7-350x350.jpg)
Immunohistochemistry of paraffin-embedded mouse brain using SUZ12 antibody (144-07786) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] SUZ12 Polyclonal Antibody (144-07786)](https://cdn.sanity.io/images/g51potcz/production/27551969b519a7ebe7ccd4e72ddb3150142b19d3-312x350.jpg)
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug SUZ12 antibody (144-07786). Western blot was performed from the immunoprecipitate using SUZ12 antibody (144-07786) at a dilition of 1:500.![Immunoprecipitation - [KO Validated] SUZ12 Polyclonal Antibody (144-07786)](https://www.raybiotech.com/files/images/Antibodies/144/144-07786/9.jpg)
Expiration:
12 months from the date of shipment when stored properly.