TRIM28 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q13263 |
| Gene Id | 10155 |
| Gene Symbols | TRIM28 |
| Protein Name / Synonyms | TRIM28, KAP1, PPP1R157, RNF96, TF1B, TIF1B, tripartite motif containing 28 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.
Dilutions
WB 1:1000 - 1:3000
IHC 1:100 - 1:200
IHC 1:100 - 1:200
Format
Calculated MW
79kDa/88kDa
Observed MW
120kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 576-835 of human TRIM28 (NP_005753.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TRIM28 antibody (144-02245) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat brain using TRIM28 antibody (144-02245) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.