TST Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q16762 |
| Gene Id | 7263 |
| Gene Symbols | TST |
| Protein Name / Synonyms | TST, RDS, thiosulfate sulfurtransferase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This is one of two neighboring genes encoding similar proteins that each contain two rhodanese domains. The encoded protein is localized to the mitochondria and catalyzes the conversion of thiosulfate and cyanide to thiocyanate and sulfite. In addition, the protein interacts with 5S ribosomal RNA and facilitates its import into the mitochondria. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:1000 - 1:2000
IP 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
33kDa
Observed MW
36kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-297 of human TST (NP_003303.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using TST antibody (144-010542) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug TST antibody (144-010542). Western blot was performed from the immunoprecipitate using TST antibody (144-010542) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.