YAP1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P46937 |
| Gene Id | 10413 |
| Gene Symbols | YAP1 |
| Protein Name / Synonyms | YAP1, COB1, YAP, YAP2, YAP65, YKI, Yes associated protein 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50
Format
Calculated MW
36kDa/48kDa/49kDa/50kDa/52kDa/53kDa/54kDa
Observed MW
72kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 155-504 of human YAP1 (NP_001123617.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using YAP1 antibody (144-01002) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.![Western blot - [KO Validated] YAP1 Polyclonal Antibody (144-01002)](https://cdn.sanity.io/images/g51potcz/production/053c90537c688c0373c4fb48cfc9b268f9772064-332x350.jpg)
Western blot analysis of extracts from normal (control) and YAP1 knockout (KO) HeLa cells, using YAP1 antibody (144-01002) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.![Western blot - [KO Validated] YAP1 Polyclonal Antibody (144-01002)](https://cdn.sanity.io/images/g51potcz/production/f7a9e7a87e57f3bedadd7a90643e2e4902eb6d15-350x350.jpg)
Immunohistochemistry of paraffin-embedded rat heart using YAP1 antibody (144-01002) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] YAP1 Polyclonal Antibody (144-01002)](https://cdn.sanity.io/images/g51potcz/production/ee2a336fc2c6911f1df6308179c72a9b76fd196b-350x350.jpg)
Immunohistochemistry of paraffin-embedded human tonsil using YAP1 antibody (144-01002) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] YAP1 Polyclonal Antibody (144-01002)](https://cdn.sanity.io/images/g51potcz/production/6979c5fce58c32c25a96333db1eb97420b9ac2be-350x350.jpg)
Immunohistochemistry of paraffin-embedded human lung cancer using YAP1 antibody (144-01002) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] YAP1 Polyclonal Antibody (144-01002)](https://cdn.sanity.io/images/g51potcz/production/80c25035b1658398f13fa8f04ca0476b24db7956-350x350.jpg)
Immunohistochemistry of paraffin-embedded human liver cancer using YAP1 antibody (144-01002) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] YAP1 Polyclonal Antibody (144-01002)](https://cdn.sanity.io/images/g51potcz/production/f3a6ae75197c0ef406425f562e9262572a4d0f39-350x350.jpg)
Immunohistochemistry of paraffin-embedded human placenta using YAP1 antibody (144-01002) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] YAP1 Polyclonal Antibody (144-01002)](https://cdn.sanity.io/images/g51potcz/production/588bedbc40d11a543005aa1dd50f3c6c8e65fbbd-350x350.jpg)
Immunohistochemistry of paraffin-embedded mouse spinal cord using YAP1 antibody (144-01002) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] YAP1 Polyclonal Antibody (144-01002)](https://cdn.sanity.io/images/g51potcz/production/4a84bf3509e0ecabf58009476b31aa6367828aab-440x550.jpg)
Immunofluorescence analysis of HeLa cells using YAP1 antibody (144-01002) at dilution of 1:100. Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] YAP1 Polyclonal Antibody (144-01002)](https://cdn.sanity.io/images/g51potcz/production/4a5bf8837b032f57c9313b7ee67b2b94ae97a2a0-440x550.jpg)
Immunofluorescence analysis of NIH/3T3 cells using YAP1 antibody (144-01002) at dilution of 1:100. Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] YAP1 Polyclonal Antibody (144-01002)](https://cdn.sanity.io/images/g51potcz/production/3a26561ffb4b56ab7488590bbe755ad7edd04f75-440x550.jpg)
Immunofluorescence analysis of PC12 cells using YAP1 antibody (144-01002) at dilution of 1:100. Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] YAP1 Polyclonal Antibody (144-01002)](https://www.raybiotech.com/files/images/Antibodies/144/144-01002/11.jpg)
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug YAP1 antibody (144-01002). Western blot was performed from the immunoprecipitate using YAP1 antibody (144-01002) at a dilition of 1:1000.![Immunoprecipitation - [KO Validated] YAP1 Polyclonal Antibody (144-01002)](https://www.raybiotech.com/files/images/Antibodies/144/144-01002/12.jpg)
Expiration:
12 months from the date of shipment when stored properly.
