Label-Based Antibody Arrays: L-Series

Combining direct antigen-labeling technology with our vast library of array-validated antibodies, RayBiotech has created our largest antibody array to date: the Human L8000 Array. With the L-Series label-based high density array platform, researchers can now detect up to 8000 proteins simultaneously, obtaining a broad, panoramic view of protein expression. Our newly expanded panel includes a wide variety of metabolic enzymes, structural proteins, epigenetic markers, neuroregulatory factors, in addition to our popular list of cytokines, growth factors, receptors, adipokines, proteases, and signaling proteins. Available on both glass slide and membrane formats, this array is ideally suited for biomarker discovery studies and exploratory screens.

Through a simple labeling process, the sample proteins are directly conjugated to biotin, eliminating the need for a second antibody to develop the array signals. In this format, unintended antibody interactions are impossible, thus eliminating limitations on the size of the array panel. Proteins binding to the array can be detected using streptavidin-HRP for chemiluminescence detection or streptavidin-conjugated fluor for fluorescence detection.

For the glass slide versions, please view our list of laser scanner specifications. If you do not have access to a scanner, RayBiotech provides slide scanning and data analysis services.

How It Works

Our RayBio® label-based arrays differ from our multiplex ELISA arrays primarily in the means of detection. Instead of using a second biotinylated antigen-specific antibody, sample proteins themselves are labeled with biotin prior to incubation with the capture antibodies.