Bovine Cytokine Array GS30

G-Series Bovine Cytokine Array 30 Kit. A combination of 3 non-overlapping arrays to semi-quantitatively measure 30 Bovine cytokines.

GSB-CAA-30-1
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Antigen Information

Gene Symbols:
ANGPT1 | CCL2 | CD40LG | CXCL10 | CXCL9 | DCN | FGF1 | FGF2 | IFNG | IGF1   View more

Assay Format

Number of Targets Detected:
30
Species Detected:
  • Bovine (cow)
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)
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Introduction

RayBio® G-Series Arrays are glass slide-based antibody arrays which allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid. Like a traditional sandwich-based ELISA, this array uses a matched pair of cytokine-specific antibodies for detection. Like the Quantibody® Multiplex ELISA Arrays, G-Series utilizes a highly sensitive and stable fluorescent readout which can be detected by most laser fluorescent scanner systems (see below for more info). After capturing the spot densities with a laser scanner, normalization of the raw data can be easily calculated by the researcher, or by a quick copy-paste into our excel-based Analysis Tool software.

This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 µl per array).

Product Features

  • High sensitivity (pg/mL to ng/mL levels)
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Test 16 samples on each slide
  • Data analysis software provides one-step computation

Target Names

Scroll over each target protein for more information
aFGF
(FGF-1)
Angiopoietin-1
bFGF
CD40 Ligand
(TNFSF5)
Decorin
  • Gene Symbol: CD40LG CD40L TNFSF5
  • Gene ID: 282387
  • Accession #: P51749
  • ELISA Kit: RayBio
GASP-1
(WFIKKNRP)
IFN alpha
IFN-beta
IFN-gamma
IGF-1
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-10
  • Gene Symbol: IL1B
  • Gene ID: 281251
  • Accession #: P09428
  • ELISA Kit: RayBio
IL-13
  • Gene Symbol: IL10
  • Gene ID: 281246
  • Accession #: P43480
  • ELISA Kit: RayBio
IL-15
IL-17A
IL-18
IL-2
  • Gene Symbol: IL18 IGIF
  • Gene ID: 281249
  • Accession #: Q9TU73
  • ELISA Kit: RayBio
IL-21
IL-36 Ra
(IL-1 F5)
IL-4
IP-10
(CXCL10)
LIF
  • Gene Symbol: LIF
  • Gene ID: 280840
  • Accession #: Q27956
  • ELISA Kit: RayBio
MCP-1
(CCL2)
MIG
(CXCL9)
MIP-1 beta
(CCL4)
NCAM-1
(CD56)
RANTES
(CCL5)
TNF alpha
VEGF-A

Application Notes

Suggested Application
  • Multiplexed Protein Detection
  • Detection of Relative Protein Expression
  • Detecting Patterns of Cytokine Expression
  • Biomarker Screening
  • Identifying Key Factors
  • Confirming Biological Process
Kit Components
  • Antibody Array Slide/s
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • 30 ml Centrifuge Tube
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with samples
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Analysis Tool Bundle Discount

70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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