Mouse Cytokine Array C3

RayBio® C-Series Mouse Cytokine Antibody Array 3 Kit. Detects 62 Mouse Cytokines. Suitable for all liquid sample types.


Antigen Information

Gene Symbols:
AXL | CCL1 | CCL11 | CCL12 | CCL17 | CCL19 | CCL2 | CCL20 | CCL24 | CCL25   View more

Assay Format

Number of Targets Detected:
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Semi-Quantitative
Solid Support:

Product Specifications

2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
CD30 Ligand
Fas Ligand
IL-1 beta
(IL-1 F2)
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
  • Gene Symbol: IL17A
  • Accession #: Q60971
IL-1 alpha
(IL-1 F1)
IL-3 R beta
Leptin R
MIP-1 alpha
MIP-1 gamma
MIP-3 beta
MIP-3 alpha
Platelet Factor 4
SDF-1 alpha
(CXCL12 alpha)
TNF alpha

Species Detected


Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C3 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis


For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Li J., Zhou Z., Zhang X., et al. Inflammatory Molecule, PSGL-1, Deficiency Activates Macrophages to Promote Colorectal Cancer Growth through NF?B Signaling. Mol Cancer Res. 2017 Apr;15(4):467-477. doi: 10.1158/1541-7786.MCR-16-0309.
    Species: Mouse
    Sample type: Serum
  2. Wang R., Yu Z., Sunchu B., et al. Rapamycin inhibits the secretory phenotype of senescent cells by a Nrf2-independent mechanism. Aging Cell. 2017 Mar 31. doi: 10.1111/acel.12587
    Species: Mouse
    Sample type: Conditioned Media
  3. Palm A., Garcia-Faroldi G., Lundberg., et al. Activated mast cells promote differentiation of B cells into effector cells. Sci Rep. 2016 Feb 5;6:20531. doi: 10.1038/srep20531
    Species: Mouse
    Sample type: Conditioned Media
  4. Ivshina M., Alexandrov IM., Vertii A., et al. CPEB Regulation of TAK1 Synthesis Mediates Cytokine Production and the Inflammatory Immune Response. Mol Cell Biol. 2015 Feb 1;35(3):610-8. doi: 10.1128/MCB.00800-14
    Species: Mouse
    Sample type: Serum
  5. Grum-Schwensen B., Klingelhofer J., Beck M., et al. S100A4-neutralizing antibody suppresses spontaneous tumor progression, pre-metastatic niche formation and alters T-cell polarization balance. BMC Cancer. 2015; 15: 44. doi: 10.1186/s12885-015-1034-2
    Species: Mouse
    Sample type: Conditioned Media
  6. Pelaez-Garcia A., Barderas R., Batlle R., et al. A proteomic analysis reveals that Snail regulates the expression of the nuclear orphan receptor Nuclear Receptor Subfamily 2 Group F Member 6 (Nr2f6) and interleukin 17 (IL-17) to inhibit adipocyte differentiation. Mol Cell Proteomics. 2015 Feb;14(2):303-15. doi: 10.1074/mcp.M114.045328.
    Species: Mouse
    Sample type: Conditioned Media
  7. Reineke LC, Lloyd RE. 2015. The stress granule protein G3BP1 recruits protein kinase R to promote multiple innate immune antiviral responses. J Virol 89:2575-2589. doi:10.1128/JVI.02791-14.
    Species: Mouse
    Sample type: Conditioned Media
  8. Vergori L., Lauret E., Gaceb A., et al. PPAR? Regulates Endothelial Progenitor Cell Maturation and Myeloid Lineage Differentiation Through a NADPH Oxidase-Dependent Mechanism in Mice. Stem Cells. 2015 Apr;33(4):1292-303. doi: 10.1002/stem.1924.
    Species: Mouse
    Sample type: Conditioned Media
  9. Yang Y., Gorzelanny C., Bauer AT., et al. Nuclear heparanase-1 activity suppresses melanoma progression via its DNA-binding affinity. Oncogene. 2015 Mar 9. doi: 10.1038/onc.2015.40.
    Species: Mouse
    Sample type: Tissue Lysate
  10. Obenauf A., Zou Y., Ji A., et al. Therapy-induced tumour secretomes promote resistance and tumour progression. Nature. 2015 Apr 16;520(7547):368-72. doi: 10.1038/nature14336.
    Species: Mouse
    Sample type: Conditioned Media
We used the assay to analyze lung tissue extracts at 500ug per membrane. We got a good signal and reproducible results. However, I would recommend using different ECL reagent for detection, the one provided with the kit is not the best.

University of Kansas Medical Center
The protocol is easy and user friendly. However, the read out proved difficult as the radiance between samples in different conditions can not be discerned clearly even when using advanced western blot readout software. Tried different exposures and did not get adequate results for a manuscript figure.

National Cancer Institute, NIH
We used the above kit to get the cytokine patterns in the secretome of the infected bone marrow derived macrophages. The kit seems to be consistent. With in vitro stimulated cells, the cytokine levels were found. We used undiluted samples in analysis. The FBS from VWR at 2% was also present in the medium.

University of Chicago
We used the AAM-CYT-3-8 Kit. With the detailed protocol manual, we could take all the necessary precautions to get consistent signals with the positive controls and we could confirm the same after repeating it for the selected individual cytokines that were deferentially unregulated

W.M. Keck Center for Transgene Research
This product is fantastic! We use to analyze the connection between irradiation dose and inflammation in mouse plasma in mice with different genetic backgrounds. The protocol is easy to follow and the results are always clear to interpret.

Roswell Park Cancer Institute
I used this array in the serum samples of deer mice and got good pictures. The images can be easily analyzed by Imagelab.

University of South Carolina
Protocol is easy to follow and convenient to use. It is time-saving.

Purdue University
I used this array to analyze some astrocyte culture-conditioned (serum-free) media (about 1 million cells/well). I didn't use the software for analysis, and it took me about a day to analyze the four membranes. I didn't dilute the media and I certainly wasn't maxing out the signal when going with all of the optional overnight incubations.

Northwestern University
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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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