Mouse Cytokine Array C3

$423.00
AAM-CYT-3-2

RayBio® C-Series Mouse Cytokine Antibody Array 3 Kit. Detects 62 Mouse Cytokines. Suitable for all liquid sample types.

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information

Axl
BLC
(CXCL13)
CD30 Ligand
(TNFSF8)
CD30
(TNFRSF8)
CD40
(TNFRSF5)
CRG-2
CTACK
(CCL27)
CXCL16
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Fas Ligand
(TNFSF6)
Fractalkine
(CX3CL1)
GCSF
GM-CSF
IFN-gamma
IGFBP-3
IGFBP-5
IGFBP-6
IL-1 beta
(IL-1 F2)
IL-10
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-17A
  • Gene Symbol: IL17A
  • Accession #: Q60971
IL-1 alpha
(IL-1 F1)
IL-2
IL-3
IL-3 R beta
IL-4
IL-5
IL-6
IL-9
KC
(CXCL1)
Leptin
Leptin R
LIX
L-Selectin
(CD62L)
Lymphotactin
(XCL1)
MCP-1
(CCL2)
MCP-5
M-CSF
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 gamma
MIP-2
MIP-3 beta
(CCL19)
MIP-3 alpha
(CCL20)
Platelet Factor 4
(CXCL4)
P-Selectin
RANTES
(CCL5)
SCF
SDF-1 alpha
(CXCL12 alpha)
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
TARC
(CCL17)
I-309
(TCA-3/CCL1)
TECK
(CCL25)
TIMP-1
TNF alpha
Thrombopoietin
(TPO)
VCAM-1
(CD106)
VEGF-A

Species Detected

Mouse

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C3 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Number of Targets Detected

62

Antigen Information

Gene Symbol:
  • AXL
  • CCL1
  • CCL11
  • CCL12
  • CCL17
  • CCL19
  • CCL2
  • CCL20
  • CCL24
  • CCL25
  • CCL27
  • CCL3
  • CCL5
  • CCL9
  • CD40
  • CSF1
  • CSF2
  • CSF2RB
  • CSF3
  • CX3CL1
  • CXCL1
  • CXCL10
  • CXCL12
  • CXCL13
  • CXCL16
  • CXCL2
  • CXCL5
  • CXCL9
  • FASLG
  • IFNG
  • IGFBP3
  • IGFBP5
  • IGFBP6
  • IL10
  • IL12A
  • IL13
  • IL17A
  • IL1A
  • IL1B
  • IL2
  • IL3
  • IL4
  • IL5
  • IL6
  • IL9
  • KITLG
  • LEP
  • LEPR
  • PF4
  • SELL
  • SELP
  • THPO
  • TIMP1
  • TNF
  • TNFRSF8
  • TNFSF8
  • TPO
  • VCAM1
  • VEGFA
  • XCL1

Product Specifications

Size:
2, 4, or 8 Sample Kit

Array/ELISA Format

Species Detected:
  • Mouse
Solid Support:
Membrane
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Semi-Quantitative
Method of Detection:
Chemiluminescence
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants
  • Cell Lysates
  • Tissue Lysates

  1. Palm A., Garcia-Faroldi G., Lundberg., et al. Activated mast cells promote differentiation of B cells into effector cells. Sci Rep. 2016 Feb 5;6:20531. doi: 10.1038/srep20531
    Species: Mouse
    Sample Type: Conditioned Media

  2. Ivshina M., Alexandrov IM., Vertii A., et al. CPEB Regulation of TAK1 Synthesis Mediates Cytokine Production and the Inflammatory Immune Response. Mol Cell Biol. 2015 Feb 1;35(3):610-8. doi: 10.1128/MCB.00800-14
    Species: Mouse
    Sample Type: Serum

  3. Grum-Schwensen B., Klingelhofer J., Beck M., et al. S100A4-neutralizing antibody suppresses spontaneous tumor progression, pre-metastatic niche formation and alters T-cell polarization balance. BMC Cancer. 2015; 15: 44. doi: 10.1186/s12885-015-1034-2
    Species: Mouse
    Sample Type: Conditioned Media

  4. Pelaez-Garcia A., Barderas R., Batlle R., et al. A proteomic analysis reveals that Snail regulates the expression of the nuclear orphan receptor Nuclear Receptor Subfamily 2 Group F Member 6 (Nr2f6) and interleukin 17 (IL-17) to inhibit adipocyte differentiation. Mol Cell Proteomics. 2015 Feb;14(2):303-15. doi: 10.1074/mcp.M114.045328.
    Species: Mouse
    Sample Type: Conditioned Media

  5. Reineke LC, Lloyd RE. 2015. The stress granule protein G3BP1 recruits protein kinase R to promote multiple innate immune antiviral responses. J Virol 89:2575-2589. doi:10.1128/JVI.02791-14.
    Species: Mouse
    Sample Type: Conditioned Media

  6. Vergori L., Lauret E., Gaceb A., et al. PPARα Regulates Endothelial Progenitor Cell Maturation and Myeloid Lineage Differentiation Through a NADPH Oxidase-Dependent Mechanism in Mice. Stem Cells. 2015 Apr;33(4):1292-303. doi: 10.1002/stem.1924.
    Species: Mouse
    Sample Type: Conditioned Media

  7. Yang Y., Gorzelanny C., Bauer AT., et al. Nuclear heparanase-1 activity suppresses melanoma progression via its DNA-binding affinity. Oncogene. 2015 Mar 9. doi: 10.1038/onc.2015.40.
    Species: Mouse
    Sample Type: Tissue Lysate

  8. Obenauf A., Zou Y., Ji A., et al. Therapy-induced tumour secretomes promote resistance and tumour progression. Nature. 2015 Apr 16;520(7547):368-72. doi: 10.1038/nature14336.
    Species: Mouse
    Sample Type: Conditioned Media

  9. Kim I., Kim I., Wen Y., et al. Vibrio vulnificus Secretes an Insulin-degrading Enzyme That Promotes Bacterial Proliferation in Vivo. J Biol Chem. 2015 Jul 24;290(30):18708-20. doi: 10.1074/jbc.M115.656306.
    Species: Mouse
    Sample Type: Serum

  10. Taka E., Mazzio E., Goodman C., et al. Anti-inflammatory effects of thymoquinone in activated BV-2 microglial cells. J Neuroimmunol. 2015 Sep 15;286:5-12. doi: 10.1016/j.jneuroim.2015.06.011.
    Species: Mouse
    Sample Type: Conditioned Media

  11. Jin H., Chen L., Zhang Z., et al. Deletion of angiotensin-converting enzyme 2 exacerbates renal inflammation and injury in apolipoprotein E-deficient mice through modulation of the nephrin and TNF-alpha-TNFRSF1A signaling. J Transl Med. 2015 Aug 6;13:255. doi: 10.1186/s12967-015-0616-8.
    Species: Mouse
    Sample Type: Tissue Lysate

  12. Li W., Wu A., Zhu S., et al. EGCG induces G-CSF expression and neutrophilia in experimental sepsis. Immunol Res. 2015 Dec;63(1-3):144-52. doi: 10.1007/s12026-015-8681-x.
    Species: Mouse
    Sample Type: Peritoneal Fluid

  13. Li W., Wu A., Zhu S., et al. EGCG induces G-CSF expression and neutrophilia in experimental sepsis. Immunol Res. 2015 Dec;63(1-3):144-52. doi: 10.1007/s12026-015-8681-x.
    Species: Mouse
    Sample Type: Conditioned Media

  14. Achyut B., Shankar A., Iskander A., et al. Bone marrow derived myeloid cells orchestrate antiangiogenic resistance in glioblastoma through coordinated molecular networks. Cancer Lett. 2015 Dec 28;369(2):416-26. doi: 10.1016/j.canlet.2015.09.004.
    Species: Mouse
    Sample Type: Tissue Lysate

  15. Karakasheva T., Waldron T., Eruslanov E., et al. CD38-Expressing Myeloid-Derived Suppressor Cells Promote Tumor Growth in a Murine Model of Esophageal Cancer. Cancer Res. 2015 Oct 1;75(19):4074-85. doi: 10.1158/0008-5472.CAN-14-3639
    Species: Mouse
    Sample Type: Conditioned Media

  16. Braig M., Pallman N., Preukschas M., Steinemann D., Hofmann W., et al. A ‘telomere-associated secretory phenotype’ cooperates with BCR-κL to drive malignant proliferation of leukemic cells. 2014 Leukemia, Pubslihed April 1, Epub Ahead of Print, doi: 10.1038/leu.2014.95
    Species: Mouse
    Sample Type: Conditioned Media

  17. Cho D., Kim M., Jeong H.l, Jeong H., Jeong M., et al. Mesenchymal stem cells reciprocally regulate the M1/M2 balance in mouse bone marrow-derived macrophages. Experimental & Molecular Medicine (2014) 46, e70; doi:10.1038/emm.2013.135
    Species: Mouse
    Sample Type: Conditioned Media

  18. Ehninger D. Tsc2 Haploinsufficiency Has Limited Effects on Fetal BrainCytokine Levels during Gestational Immune Activation. Autism Research and Treatment. Volume 2014, Article ID 761279, 7 pages http://dx.doi.org/10.1155/2014/761279
    Species: Mouse
    Sample Type: Tissue Lysate

  19. Llorens-Martin M., et al. Peripherally triggered and GSK-3β-driven brain inflammation differentially skew adult hippocampal neurogenesis, behavioral pattern separation and microglial activation in response to ibuprofen. Translational Psychiatry (2014) 4, e463; doi:10.1038/tp.2014.92; published online 14 October 2014
    Species: Mouse
    Sample Type: Tissue Lysate

  20. Palmi C., et al. Cytoskeletal Regulatory Gene Expression and Migratory Properties of B Cell Progenitors are Affected by the ETV6-RUNX1 Rearrangement Mol Cancer Res molcanres.0056.2014; Published OnlineFirst July 24, 2014; doi: 10.1158/1541-7786.MCR-14-0056-T
    Species: Mouse
    Sample Type: Conditioned Media

  21. Pavlides S., et al. Caveolin-1 regulates the anti-atherogenic properties of macrophages. Cell Tissue Res (2014) 358:821-831. DOI 10.1007/s00441-014-2008-4
    Species: Mouse
    Sample Type: Conditioned Media

  22. Price P., et al. Chemokine (C-C motif) receptor 1 (CCR1) is required for efficient recruitment of neutrophils during respiratory infection with Modified Vaccinia virus Ankara J. Virol. published ahead of print 9 July 2014 , doi:10.1128/JVI.01524-14
    Species: Mouse
    Sample Type: Conditioned Media

  23. Szabolcsi V., Celio M. De novo Expression of Parvalbumin in Ependymal Cells in Response to Brain Injury Promotes Ependymal Remodeling and Wound Repair. GLIA 2014, epub ahead of print. DOI: 10.1002/glia.22768
    Species: Mouse
    Sample Type: Conditioned Media

  24. Taka E., Mazzio E., Goodman C., Reeams R., Soliman K. Anti- inflammatory effects of thymoquinone in LPS- stimulated BV-2 murine microglia cells. The FASEB Journal (April 2014) vol. 28 no. 1 Supplement 730.2
    Species: Mouse
    Sample Type: Conditioned Media

  25. Venmar K., Carter K., Hwang D., Dozier A., Fingleton B. IL-4 receptor ILR4α regulates metastatic colonization by mammary tumors through multiple signaling pathways. Cancer Research Published OnlineFirst June 19, 2014; doi: 10.1158/0008-5472.CAN-14-0093
    Species: Mouse
    Sample Type: Conditioned Media

  26. Vilatla M., et al. Recruitment of Circulating Breast Cancer Cells Is Stimulated by Radiotherapy. Cell Available online 10 July 2014. http://dx.doi.org/10.1016/j.celrep.2014.06.011
    Species: Mouse
    Sample Type: Conditioned Media

  27. Ha CT, Li X-H, Fu D, Moroni M, Fisher C, et al. (2014) Circulating Interleukin-18 as a Biomarker of Total-Body Radiation Exposure in Mice, Minipigs, and Nonhuman Primates (NHP). PLoS ONE 9(10): e109249. doi:10.1371/journal.pone.0109249
    Species: Mouse
    Sample Type: Serum

  28. Cyclophilin D Extramitochondrial Signaling Controls Cell Cycle Progression and Chemokine-Directed Cell Motility
    Species: Mouse
    Sample Type: Conditioned Media

  29. Orientia tsutsugamushi subverts dendritic cell functions by escaping from autophagy and impairing their migration
    Species: Mouse
    Sample Type: Conditioned Media

  30. Orientia tsutsugamushi subverts dendritic cell functions by escaping from autophagy and impairing their migration
    Species: Mouse
    Sample Type: Conditioned Media

  31. Benabid R., et al. Neutrophil Elastase Modulates Cytokine Expression: CONTRIBUTION TO HOST DEFENSE AGAINST PSEUDOMONAS AERUGINOSA-INDUCED PNEUMONIA. THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 287, NO. 42, pp. 34883-34894, October 12, 2012; DOI 10.1074/jbc.M112.361352
    Species: Mouse
    Sample Type: BAL Fluid

  32. Bethunaickan R, Sahu R, Liu Z, et al. Anti-TNF treatment of IFN induced lupus nephritis reduces the renal macrophage response but does not alter glomerular immune complex formation. Arthritis and rheumatism 2012;64(10):3399-3408. doi:10.1002/art.34553.
    Species: Mouse
    Sample Type: Tissue Lysate

  33. Bobrie A., Krumeich S., Reyal F., et al. Rab27a supports exosome-dependent and -independent mechanisms that modify the tumor microenvironment and can promote tumor progression. Cancer Res. 2012 Oct 1;72(19):4920-30. doi: 10.1158/0008-5472.CAN-12-0925
    Species: Mouse
    Sample Type: Conditioned Media

  34. Goncharova V, Serobyan N, Iizuka S, et al. Hyaluronan Expressed by the Hematopoietic Microenvironment Is Required for Bone Marrow Hematopoiesis . The Journal of Biological Chemistry 2012;287(30):25419-25433. doi:10.1074/jbc.M112.376699.
    Species: Mouse
    Sample Type: Conditioned Media

  35. Li X, Sterling JA, Fan K-H, et al. Loss of TGF-β Responsiveness in Prostate Stromal Cells Alters Chemokine Levels and Facilitates the Development of Mixed Osteoblastic/Osteolytic Bone Lesions. Molecular cancer research : MCR. 2012;10(4):494-503. doi:10.1158/1541-7786.MCR-11-0506.
    Species: Mouse
    Sample Type: Conditioned Media

  36. McCurdy CE, Schenk S, Holliday MJ, et al. Attenuated Pik3r1 Expression Prevents Insulin Resistance and Adipose Tissue Macrophage Accumulation in Diet-Induced Obese Mice. Diabetes 2012;61(10):2495-2505. doi:10.2337/db11-1433.
    Species: Mouse
    Sample Type: Conditioned Media

  37. Owens P, Pickup MW, Novitskiy SV, et al. Disruption of bone morphogenetic protein receptor 2 (BMPR2) in mammary tumors promotes metastases through cell autonomous and paracrine mediators. Proceedings of the National Academy of Sciences of the United States of America 2012;109(8):2814-2819. doi:10.1073/pnas.1101139108.
    Species: Mouse
    Sample Type: Tissue Lysate

  38. Pequeuz C., Raymond-Letron I., Blacher S., et al. Stromal Estrogen Receptor-a Promotes Tumor Growth by Normalizing an Increased Angiogenesis. Cancer Res. 2012 Jun 15;72(12):3010-9. doi: 10.1158/0008-5472.CAN-11-3768
    Species: Mouse
    Sample Type: Tissue Lysate

  39. Roth W., Kumar V., Beer HD., et al. Keratin 1 maintains skin integrity and participates in an inflammatory network in skin through interleukin-18. J Cell Sci. 2012 Nov 15;125(Pt 22):5269-79. doi: 10.1242/jcs.116574
    Species: Mouse
    Sample Type: Cell Lysate

  40. Roth W., Kumar V., Beer HD., et al. Keratin 1 maintains skin integrity and participates in an inflammatory network in skin through interleukin-18. J Cell Sci. 2012 Nov 15;125(Pt 22):5269-79. doi: 10.1242/jcs.116574
    Species: Mouse
    Sample Type: Conditioned Media

  41. Zhong M, et al. L-Selectin and P-Selectin Are Novel Biomarkers of Cervicovaginal Inflammation for Preclinical Mucosal Safety Assessment of Anti-HIV-1 Microbicide. Antimicrobial Agents and Chemotherapy. 2012 Jun;56(6):3121-32. doi: 10.1128/AAC.05950-11
    Species: Mouse
    Sample Type: Cervical-Vaginal Fluid

  42. Li W., Zhu S., Zhang Y., et al. Use of Animal Model of Sepsis to Evaluate Novel Herbal Therapies. J Vis Exp. 2012; (62): 3926. doi: 10.3791/3926
    Species: Mouse
    Sample Type: Serum

  43. Sun Y., Yin M., Zhang L., Pan J. Characterization of the cytokine expression profiles of the aorta and liver of young tumor necrosis factor alpha mutant mice. Mol Cell Biochem. 2012 Jul;366(1-2):59-67. doi: 10.1007/s11010-012-1283-1
    Species: Mouse
    Sample Type: Tissue Lysate

  44. Sun Y., Yin M., Zhang L., Pan J. Characterization of the cytokine expression profiles of the aorta and liver of young tumor necrosis factor alpha mutant mice. Mol Cell Biochem. 2012 Jul;366(1-2):59-67. doi: 10.1007/s11010-012-1283-1
    Species: Mouse
    Sample Type: Tissue Lysate

  45. Kulkarni S., Cary L., Gambles K., et al. Gamma-tocotrienol, a radiation prophylaxis agent, induces high levels of granulocyte colony-stimulating factor. Int Immunopharmacol. 2012 Dec;14(4):495-503. doi: 10.1016/j.intimp.2012.09.001
    Species: Mouse
    Sample Type: Serum

  46. Rudilla F., Fayolle C., Casares N., et al. Combination of a TLR4 ligand and anaphylatoxin C5a for the induction of antigen-specific cytotoxic T cell responses. Vaccine. 2012 Apr 16;30(18):2848-58. doi: 10.1016/j.vaccine.2012.02.052.
    Species: Mouse
    Sample Type: Conditioned Media

  47. Kang TW., et al. Senescence surveillance of pre-malignant hepatocytes limits liver cancer development. Nature. 2011 Nov 9;479(7374):547-51. doi: 10.1038/nature10599.
    Species: Mouse
    Sample Type: Conditioned Media

  48. Kang TW., et al. Senescence surveillance of pre-malignant hepatocytes limits liver cancer development. Nature. 2011 Nov 9;479(7374):547-51. doi: 10.1038/nature10599.
    Species: Mouse
    Sample Type: Tissue Lysate

  49. Medina-Echeverz, José, et al. "Successful colon cancer eradication after chemoimmunotherapy is associated with profound phenotypic change of intratumoral myeloid cells." The Journal of Immunology 186.2 (2011): 807-815.

  50. Zhang, Quanshun, et al. "MyD88‐dependent pathway is essential for the innate immunity to Enterocytozoon bieneusi." Parasite immunology 33.4 (2011): 217-225.

  51. Bradford, Shayna T., et al. "Nicotine aggravates the brain postischemic inflammatory response." American Journal of Physiology-Heart and Circulatory Physiology 300.4 (2011): H1518-H1529.

  52. Henn, Anja, Susanne Kirner, and Marcel Leist. "TLR2 hypersensitivity of astrocytes as functional consequence of previous inflammatory episodes." The Journal of Immunology 186.5 (2011): 3237-3247.

  53. Hannigan, Adele, et al. "Lymphocyte deficiency limits Epstein-Barr virus latent membrane protein 1 induced chronic inflammation and carcinogenic pathology in vivo." Molecular cancer 10.1 (2011): 11.

  54. Hannigan, Adele, et al. "Lymphocyte deficiency limits Epstein-Barr virus latent membrane protein 1 induced chronic inflammation and carcinogenic pathology in vivo." Molecular cancer 10.1 (2011): 11.

  55. Acres B, Gantzer M, Remy C, Futin N, Accart N, et al. Fusokine Interleuikin-2/Interleukin-18, a Novel Potent Innate and Adaptive Immune Stimulator with Decreased Toxicity. Cancer Res. 2005;65(20):9536-9546.
    Species: Mouse
    Sample Type: Conditioned Media

  56. Li, H et al. IL-1 receptor-associated kinase M is a central regulator of osteoclast differentiation and activation. IL-1 receptor-associated kinase M is a central regulator of osteoclast differentiation and activation. IL-1 receptor-associated kinase M is a central regulator of osteoclast differentiation and activation. J. Exp. Med. Volume 201, Issue 7, Pages 1169-77, Apr 2005
    Species: Mouse
    Sample Type: Conditioned Media

  57. Zhou Q, Desta T, Fenton M, Graves DT, Amar S. Cytokine Profiling of Macrophages Exposed to Porphyromonas gingivalis, Its Lipopolysaccharide, or Its FimA Protein. Infect Immun. 2005;73(2):935-943.
    Species: Mouse
    Sample Type: Conditioned Media

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