The RayBiotech Mouse IL-6 ELISA Kit is a pre-coated immunoassay for the measurement of Interleukin 6 in mouse cell culture supernatants, plasma, serum, and lysate samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Sensitivity | 2 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 2 pg/ml - 600 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P08505 |
| Gene Id | 16193 |
| Gene Symbols | IL6, IL-6 |
| Protein Name / Synonyms | Interleukin-6 (IL-6 / IL6) (B-cell hybridoma growth factor) (Interleukin HP-1) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cardiovascular Disease, Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
The RayBio® Mouse IL-6 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Interleukin 6 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm
Interleukin 6 is a pleiotropic cytokine that regulates cell growth and differentiation of various tissues, and plays an important role in the immune response and acute phase reactions. It acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate an immune response, e.g., during infection and after trauma, especially burns or other tissue damage leading to inflammation. IL-6's role as an anti-inflammatory cytokine is mediated through its inhibitory effects on TNF-α and IL-1, and activation of IL-1Rα and IL-10.
The RayBiotech Mouse IL-6 ELISA Kit is a pre-coated immunoassay for the measurement of Interleukin 6 in mouse cell culture supernatants, plasma, serum, and lysate samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Mouse IL-6 ELISA Kit Standard Curve
Mouse IL-6 ELISA Kit Standard Curve
I used it on tissue lysate, while my samples were not highly concentrated, I did not get values for my control group.
Review by Yasmin, from University of Cincinnati, on Apr 27, 2026
Great kit and cost effective. Got good results.
Review by Simant, from Vellore institute of technology, on May 22, 2025
Recently used the IL-6 ELISA kit for quantifying interleukin-6 (IL-6) levels in cachexia mouse plasma samples. The kit came with all necessary reagents, including pre-coated 96-well plates, standards, detection antibodies, and substrates, which minimized preparation time and potential errors. The IL-6 ELISA kit protocol was straightforward, and all the assay incubations were at RT. The dilutions (2-fold) worked good as suggested in the protocol. Will order this kit for our experiments further.
Review by Neeraj Kumar Chauhan, from University of Oklahoma health sciences center, on Oct 11, 2024
We've been looking for an ELISA kit that detects IL-6 in tissue lysates for ages. We used this kit with mouse brain tissue homogenized in a lysis buffer containing 0.25% SDS and diluted the samples 1:5 as recommended by the manufacturer protocol and got beautiful results.
Review by Felecia, from University of Illinois at Chicago, on Jul 26, 2024
I recently had the opportunity to use the IL-6 ELISA kit for quantifying interleukin-6 (IL-6) levels in mouse skin tissue samples. From the outset, the IL-6 ELISA kit was straightforward and user-friendly. The kit came with all necessary reagents, including pre-coated 96-well plates, standards, detection antibodies, and substrates, which minimized preparation time and potential errors.
Review by Ayaz, from Western University of Health Science, on Jul 21, 2024
This IL-6 ELISA Kit is highly recommended for measuring IL-6 in inflammatory research studies due to its exceptional sensitivity, reproducibility, specificity, and dynamic range. I obtained good results when using it for mouse skin.
Review by Ayaz, from Western University of Health Science, on Mar 15, 2024
Very simple kit to use, tricky with mouse plasma samples though
Review by Evan, from BCM, on Jun 24, 2022
Elisa Mouse IL-6, I tested some plasma samples, and I was not able to detect any IL-6 even after incubating overnight at 4C
Review by Francina, from University of Michigan, on Oct 31, 2021
I used this kit to quantify IL-6 levels in whole brain lysates. Protocol is clear and easy, the assay works well
Review by Miriam, from Cold Spring Harbor Laboratory, on Jun 22, 2021
I analyzed mouse plasma and brain tissue with this ELISA. The directions were clear and easy to follow. No problems were noted. Would use again!
Review by Samantha Garcia, from University of Mary Washington, on Oct 21, 2020
I have used this Elisa kit, its quite affordable considering the amount of time saved and data generated from it. I have even compared IL-6 ELISA data to IL-6 luminex data (from the same samples) and they are relatively similar.
Review by daniel yun, from NanoBST, on Oct 13, 2020
I used this kit with LPS-inflammed RAW264.7 cells supernatants. It was very sensitive! The instructions were clear and detailed and the kit well assembled. I also liked that you can use just half a plate and keep the other strips for another time. I would strongly recommend.
Review by Pauline Morin, from University of Arkansas, on Sep 23, 2020
I used this ELISA kit to measure IL-6 in mouse serum. While this was not the easiest ELISA to perform, the instructions were simple enough to follow.
Review by Joe, from Emory University, on Jun 7, 2020
Protocol was very easy to follow and very reliable. Cost effective kit!
Review by daniel yun, from NanoBST, on Apr 20, 2020
I tested mouse serum. I did not measure any signals.
Review by Julia, from FDA, on Mar 17, 2020
The kit was packaged in an organized and clear manner, and it was easy to walk through each step of the procedure. I used the mouse IL-6, VEGF, and TNF-alpha kits for plasma as well as homogenized muscle samples. Each worked well, and I would be happy to use them again.
Review by Louis Hinkle, from Houston Methodist Research Institute, on Jan 26, 2020
I used this kit to analyze mouse serum samples and it did exactly what it is supposed to do. I highly recommend this product!
Review by NAfanasiev, from BTS Research, on Jan 9, 2020
