Mouse Cytokine Array C2000

RayBio® C-Series Mouse Cytokine Antibody Array 2000. A combination of RayBio® Mouse Cytokine Antibody Array C3, C4, & C5 Kit. Detects 144 Mouse Cytokines. Suitable for all liquid sample types.

$1,036.26
AAM-CYT-2000
+

Antigen Information

Gene Symbols:
ACE | AGER | AREG | AXL | CCL1 | CCL11 | CCL12 | CCL17 | CCL19 | CCL2   View more

Assay Format

Number of Targets Detected:
144
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
4-1BB
(TNFRSF9/CD137)
6Ckine
(CCL21)
ACE
ALK-1
Amphiregulin
Axl
bFGF
BLC
(CXCL13)
Cardiotrophin-1
(CT-1)
CD27
(TNFRSF7)
CD27 Ligand
(TNFSF7)
CD30 Ligand
(TNFSF8)
CD30
(TNFRSF8)
CD36
(SR-B3)
CD40
(TNFRSF5)
CD40 Ligand
(TNFSF5)
Chordin
CRG-2
CTACK
(CCL27)
CTLA-4
(CD152)
CXCL16
Decorin
DKK-1
CD26
(DPPIV)
Dtk
E-Cadherin
EGF
Endoglin
(CD105)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Epigen
Epiregulin
E-Selectin
Fas Ligand
(TNFSF6)
Fc gamma RIIB
(CD32b)
Flt-3 Ligand
Fractalkine
(CX3CL1)
Galectin-1
GCSF
GITR
(TNFRSF18)
GITR Ligand
(TNFSF18)
GM-CSF
Granzyme B
Gas 1
Gas 6
HAI-1
HGF
HGFR
ICAM-1
(CD54)
IFN-gamma
IGFBP-2
IGFBP-3
IGFBP-5
IGFBP-6
IGF-1
IGF-2
IL-1 beta
(IL-1 F2)
IL-1 R4
(ST2)
  • Gene Symbol: IL1RL1
  • Accession #: Q05208
IL-10
IL-11
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-15
IL-17A
  • Gene Symbol: IL17A
  • Accession #: Q60971
IL-17B
IL-17 RB
IL-17E
(IL-25)
IL-17F
IL-1 alpha
(IL-1 F1)
IL-1 RA
(IL-1 F3)
IL-2
IL-2 R alpha
IL-20
IL-21
IL-28A
(IFN-lambda 2)
IL-3
IL-3 R beta
IL-4
IL-5
IL-6
IL-6 R
IL-7
IL-9
I-TAC
(CXCL11)
JAM-A
(CD321/F11R)
KC
(CXCL1)
Leptin
Leptin R
LIX
L-Selectin
(CD62L)
Lungkine
(CXCL15)
Lymphotactin
(XCL1)
MAdCAM-1
MCP-1
(CCL2)
MCP-5
M-CSF
MDC
(CCL22)
MFG-E8
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 gamma
MIP-2
MIP-3 beta
(CCL19)
MIP-3 alpha
(CCL20)
MMP-2
MMP-3
Neprilysin
Osteopontin
(SPP1)
Osteoprotegerin
(TNFRSF11B)
Pentraxin-3
(TSG-14)
Platelet Factor 4
(CXCL4)
Prolactin
Pro-MMP-9
P-Selectin
RAGE
RANTES
(CCL5)
Resistin
SCF
SDF-1 alpha
(CXCL12 alpha)
Sonic Hedgehog N-Terminal
(Shh-N)
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
TACI
(TNFRSF13B)
TARC
(CCL17)
I-309
(TCA-3/CCL1)
TECK
(CCL25)
TCK-1
(CXCL7)
TIMP-1
TIMP-2
TNF alpha
Thrombopoietin
(TPO)
TRANCE
(TNFSF11)
TREM-1
TROY
(TNFRSF19)
TSLP
TWEAK
(TNFSF12)
TWEAK R
(TNFRSF12)
VCAM-1
(CD106)
VEGF-A
VEGFR1
VEGFR2
VEGFR3
VEGF-D
  • Gene Symbol: FIGF
  • Accession #: A2AIH3

Species Detected

Mouse

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C2000 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Wang R., Yu Z., Sunchu B., et al. Rapamycin inhibits the secretory phenotype of senescent cells by a Nrf2-independent mechanism. Aging Cell. 2017 Mar 31. doi: 10.1111/acel.12587
    Species: Mouse
    Sample type: Conditioned Media
  2. Chang WJ., Song LJ, Yi T., et al. Early activated hepatic stellate cell-derived molecules reverse acute hepatic injury. World Journal of Gastroenterology. Published January 2015, Man#14271
    Species: Mouse
    Sample type: Conditioned Media
  3. Obenauf A., Zou Y., Ji A., et al. Therapy-induced tumour secretomes promote resistance and tumour progression. Nature. 2015 Apr 16;520(7547):368-72. doi: 10.1038/nature14336.
    Species: Mouse
    Sample type: Conditioned Media
  4. Files D., Liu C., Seeds M., Morris P., Delbono O. Neutrophilic Lung Injury Is Attenuated By Therapeutic Exercise In Young, But Not Old Mice With Ards. Am J Respir Crit Care Med 189;2014:A3988. [Poster abstract]
    Species: Mouse
    Sample type: BAL Fluid
  5. Takahashi, Kazue, Patience Moyo, and Lorencia Chigweshe. "Efficacy of Recombinant Chimeric Lectins, Consisting of Mannose Binding Lectin and L-ficolin, against Influenza A Viral Infection in Mouse Model Study." Virus Research. ScienceDirect, 26 Dec. 2013. Web.
    Species: Mouse
    Sample type: Tissue Lysate
  6. Owens, Philip, et al. "Bone morphogenetic proteins stimulate mammary fibroblasts to promote mammary carcinoma cell invasion." PloS one 8.6 (2013): e67533.
    Species: Mouse
    Sample type: Conditioned Media
  7. Bobrie A., Krumeich S., Reyal F., et al. Rab27a supports exosome-dependent and -independent mechanisms that modify the tumor microenvironment and can promote tumor progression. Cancer Res. 2012 Oct 1;72(19):4920-30. doi: 10.1158/0008-5472.CAN-12-0925
    Species: Mouse
    Sample type: Conditioned Media
  8. Owens P, Pickup MW, Novitskiy SV, et al. Disruption of bone morphogenetic protein receptor 2 (BMPR2) in mammary tumors promotes metastases through cell autonomous and paracrine mediators. Proceedings of the National Academy of Sciences of the United States of America 2012;109(8):2814-2819. doi:10.1073/pnas.1101139108.
    Species: Mouse
    Sample type: Tissue Lysate
  9. Ge, Qian, et al. "Adipokines identified as new downstream targets for adiponectin: lessons from adiponectin-overexpressing or-deficient mice." American Journal of Physiology-Endocrinology and Metabolism 301.2 (2011): E326-E335.
    Species: Mouse
    Sample type: Conditioned Media
  10. Li, Jing, et al. "Hyperglycemia in apolipoprotein E-deficient mouse strains with different atherosclerosis susceptibility." Cardiovascular diabetology 10.1 (2011): 117.
    Species: Mouse
    Sample type: Plasma

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Analysis Tool Bundle Discount

Specify Size
1 x $1,036.26
+
1 x $234.32 $70.30

70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Total list price $1,270.58
Price for all $1,106.56

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.