Mouse Cytokine Array C2000

RayBio® C-Series Mouse Cytokine Antibody Array 2000. A combination of RayBio® Mouse Cytokine Antibody Array C3, C4, & C5 Kit. Detects 144 Mouse Cytokines. Suitable for all liquid sample types.

$1,036.26  
AAM-CYT-2000-2
+

Antigen Information

Gene Symbols:
ACE | AGER | AREG | AXL | CCL1 | CCL11 | CCL12 | CCL17 | CCL19 | CCL2   View more

Assay Format

Number of Targets Detected:
144
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
4-1BB
(TNFRSF9/CD137)
6Ckine
(CCL21)
ACE
ALK-1
Amphiregulin
Axl
bFGF
BLC
(CXCL13)
Cardiotrophin-1
(CT-1)
CD27
(TNFRSF7)
CD27 Ligand
(TNFSF7)
CD30 Ligand
(TNFSF8)
CD30
(TNFRSF8)
CD36
(SR-B3)
CD40
(TNFRSF5)
CD40 Ligand
(TNFSF5)
Chordin
CRG-2
CTACK
(CCL27)
CTLA-4
(CD152)
CXCL16
Decorin
DKK-1
CD26
(DPPIV)
Dtk
E-Cadherin
EGF
Endoglin
(CD105)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Epigen
Epiregulin
E-Selectin
Fas Ligand
(TNFSF6)
Fc gamma RIIB
(CD32b)
Flt-3 Ligand
Fractalkine
(CX3CL1)
Galectin-1
GCSF
GITR
(TNFRSF18)
GITR Ligand
(TNFSF18)
GM-CSF
Granzyme B
Gas 1
Gas 6
HAI-1
HGF
HGFR
ICAM-1
(CD54)
IFN-gamma
IGFBP-2
IGFBP-3
IGFBP-5
IGFBP-6
IGF-1
IGF-2
IL-1 beta
(IL-1 F2)
IL-1 R4
(ST2)
  • Gene Symbol: IL1RL1
  • Accession #: Q05208
IL-10
IL-11
IL-12 p40/p70
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-15
IL-17A
  • Gene Symbol: IL17A
  • Accession #: Q60971
IL-17B
IL-17 RB
IL-17E
(IL-25)
IL-17F
IL-1 alpha
(IL-1 F1)
IL-1 RA
(IL-1 F3)
IL-2
IL-2 R alpha
IL-20
IL-21
IL-28A
(IFN-lambda 2)
IL-3
IL-3 R beta
IL-4
IL-5
IL-6
IL-6 R
IL-7
IL-9
I-TAC
(CXCL11)
JAM-A
(CD321/F11R)
KC
(CXCL1)
Leptin
Leptin R
LIX
L-Selectin
(CD62L)
Lungkine
(CXCL15)
Lymphotactin
(XCL1)
MAdCAM-1
MCP-1
(CCL2)
MCP-5
M-CSF
MDC
(CCL22)
MFG-E8
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 gamma
MIP-2
MIP-3 beta
(CCL19)
MIP-3 alpha
(CCL20)
MMP-2
MMP-3
Neprilysin
Osteopontin
(SPP1)
Osteoprotegerin
(TNFRSF11B)
Pentraxin-3
(TSG-14)
Platelet Factor 4
(CXCL4)
Prolactin
Pro-MMP-9
P-Selectin
RAGE
RANTES
(CCL5)
Resistin
SCF
SDF-1 alpha
(CXCL12 alpha)
Sonic Hedgehog N-Terminal
(Shh-N)
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
TACI
(TNFRSF13B)
TARC
(CCL17)
I-309
(TCA-3/CCL1)
TECK
(CCL25)
TCK-1
(CXCL7)
TIMP-1
TIMP-2
TNF alpha
Thrombopoietin
(TPO)
TRANCE
(TNFSF11)
TREM-1
TROY
(TNFRSF19)
TSLP
TWEAK
(TNFSF12)
TWEAK R
(TNFRSF12)
VCAM-1
(CD106)
VEGF-A
VEGFR1
VEGFR2
VEGFR3
VEGF-D
  • Gene Symbol: FIGF
  • Accession #: A2AIH3

Species Detected

Mouse

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C2000 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Obenauf A., Zou Y., Ji A., et al. Therapy-induced tumour secretomes promote resistance and tumour progression. Nature. 2015 Apr 16;520(7547):368-72. doi: 10.1038/nature14336.
    Species: Mouse
    Sample type: Conditioned Media (Stroma derived cytokines)
  2. Himburg H., Doan P., Quarmyne M., et al. Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms. Nat Med. 2017 Jan;23(1):91-99. doi: 10.1038/nm.4251.
    Species: Mouse
    Sample type: Bone Marrow Aspirate (Bone marrow supernates)
  3. Schneider R., Mullally A., Dugourd A., et al. Gli1+ Mesenchymal Stromal Cells Are a Key Driver of Bone Marrow Fibrosis and an Important Cellular Therapeutic Target. Cell Stem Cell . 2017 Jun 1;20(6):785-800.e8. doi: 10.1016/j.stem.2017.03.008. Epub 2017 Apr 27.
    Species: Mouse
    Sample type: Conditioned Media (Ckit+ Stem cells)
  4. Files D., Liu C., Seeds M., Morris P., Delbono O. Neutrophilic Lung Injury Is Attenuated By Therapeutic Exercise In Young, But Not Old Mice With Ards. Am J Respir Crit Care Med 189;2014:A3988. [Poster abstract]
    Species: Mouse
    Sample type: BAL Fluid (Brochoalveolar fluid (BAL), Lung Tissues, and/or Serum)
  5. Owens P, Pickup MW, Novitskiy SV, et al. Disruption of bone morphogenetic protein receptor 2 (BMPR2) in mammary tumors promotes metastases through cell autonomous and paracrine mediators. Proceedings of the National Academy of Sciences of the United States of America 2012;109(8):2814-2819. doi:10.1073/pnas.1101139108.
    Species: Mouse
    Sample type: Tissue Lysate (Mammary tumor lysates)
  6. Bobrie A., Krumeich S., Reyal F., et al. Rab27a supports exosome-dependent and -independent mechanisms that modify the tumor microenvironment and can promote tumor progression. Cancer Res. 2012 Oct 1;72(19):4920-30. doi: 10.1158/0008-5472.CAN-12-0925
    Species: Mouse
    Sample type: Conditioned Media (Scr, 4T1 cells)
  7. El Behi, Mohamed, et al. "An essential role for decorin in bladder cancer invasiveness." EMBO molecular medicine 5.12 (2013): 1835-1851.
    Species: Mouse
    Sample type: Conditioned Media (MB49 or MB49-I cells)
  8. Wang R., Yu Z., Sunchu B., et al. Rapamycin inhibits the secretory phenotype of senescent cells by a Nrf2-independent mechanism. Aging Cell. 2017 Mar 31. doi: 10.1111/acel.12587
    Species: Mouse
    Sample type: Conditioned Media (Mouse embryonic Fibroblasts)
  9. Li, Jing, et al. "Hyperglycemia in apolipoprotein E-deficient mouse strains with different atherosclerosis susceptibility." Cardiovascular diabetology 10.1 (2011): 117.
    Species: Mouse
    Sample type: Plasma (atherosclerosis susceptibility in B6.apoE-/- and BALB.apoE-/- mice on different diets)
  10. Ge, Qian, et al. "Adipokines identified as new downstream targets for adiponectin: lessons from adiponectin-overexpressing or-deficient mice." American Journal of Physiology-Endocrinology and Metabolism 301.2 (2011): E326-E335.
    Species: Mouse
    Sample type: Conditioned Media
Sensitivity of this array is good. however, the membrane was labelled manually (by pen) and faced membrane to membrane signal variation. Some membranes has to washed more to get clear signal than others.

National Cancer Institute (NCI)
Write A Review
U.S. Non-U.S.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Risk-Free Guarantee

Amazon Gift Card Program

C-Series Testing Service C-Series Testing Service

Related Products Related Products