COVID-19 Binding Assays

SARS-CoV-2 Inhibitor Screening ELISA kits

COVID-19 binding assays enable rapid, high throughput, and in vitro screening of potential inhibitors of Spike-receptor complexes that facilitate SARS-CoV-2 viral entry. These enzyme-linked immunosorbent assays (ELISAs) use a 96-well plate and produce a colorimetric read-out for easy data interpretation. RayBiotech offers binding assays for studying interactions between the SARS-CoV-2 Spike protein (wild-type & variants) and key host cell receptors, including angiotensin I converting enzyme 2 (ACE2), neuropilin-1 (NRP1), and tyrosine-protein kinase receptor UFO (AXL). RayBio® COVID-19 binding assays are a perfect solution for laboratories with a limited budget. In addition, the kits can be handled safely at biosafety level 1 (BSL1), although the BSL requirements may change based on the sample type being tested. Spike-ACE2 inhibitors can also be screened in cell culture with our COVID-19 Pseudovirus Service.

ACE2 and SARS-CoV-2 Spike Protein Binding
Viral entry is mediated by the interaction between the SARS-CoV-2 Spike protein and the human ACE2 receptor.

How It Works

Steps showing the process of the SARS-CoV-2 binding assay
Studying the interaction between the SARS-CoV-2 Spike Subunit 1 (Spike S1) and the extracellular domain of the human Neuropilin-1 (NRP1) receptor in the presence of a potential inhibitor with the RayBio® COVID-19 Spike-CendR-NRP1 Binding Assay Kit (cat no. CoV-CendR-NRP1).

Kit Features

  • High throughput: Screen hundreds of potential inhibitors of the Spike-receptor complexes simultaneously
  • Rapid: Get results in 5 hours!
  • Simple to use: Colorimetric assay requires a common microplate reader capable of measuring absorbance at 450 nm
  • Compatible with various sample types: Small molecules, antibodies, sera, and peptides

Contents of Kit

  • 96-well microplate (12 strips x 8 wells) pre-coated with the natural ligand for the recombinant protein
  • Wash buffer concentrate (20x)
  • Assay diluent (5x)
  • Recombinant protein
  • Detection antibody or HRP-conjugated detection antibody specific to the recombinant protein
  • HRP-conjugated anti-detection antibody (included in some kits)
  • TMB One-Step Substrate Reagent
  • Stop Solution

Full testing service is available. E-mail [email protected] to learn more.

Spike Variants

Multiple variants of the SARS-CoV-2 Spike protein compared to the wild-type "Wuhan" strain (Accession no. QHD43416) have emerged since the COVID-19 pandemic began. RayBiotech offers inhibitor screening ELISA kits with Spike variants that have mutations within the receptor binding domain (RBD). The binding of the RBD to ACE2 enables viral entry. Our Spike variant binding assays include:

B.1.1.7 Variant (U.K. Strain)
Spike protein mutations include a deletion at HV69-70 and two amino acid mutations: N501Y, D614G.

N501Y Variant (Common in U.K, Brazil & South Africa)
Spike protein with one amino acid mutation: N501Y.

E484K Variant (Common in Brazil & South Africa)
Spike protein with one amino acid mutation: E484K.

B.1.351 Variant (South Africa strain)
Spike protein with three amino acid mutations: K417N, E484K, N501Y.

P.1 Variant (Brazil strain)
Spike protein with three amino acid mutations: K417T, E484K, N501Y.

D614G Variant (U.K strain)
Spike protein with one amino acid mutation: D614G.

Citations

  1. Boroduske, Anete et al. "Wild Sambucus nigra L. from north-east edge of the species range: A valuable germplasm with inhibitory capacity against SARS-CoV2 S-protein RBD and hACE2 binding in vitro." Industrial crops and products vol. 165 (2021): 113438. doi:10.1016/j.indcrop.2021.113438 [view publication]
    • Product: CoV-SACE2-1
    • Sample Type: Fruit extract
  2. Olaleye, Omonike A et al. "Discovery of Clioquinol and analogues as novel inhibitors of Severe Acute Respiratory Syndrome Coronavirus 2 infection, ACE2 and ACE2 - Spike protein interaction in vitro." Heliyon vol. 7,3 (2021): e06426. doi:10.1016/j.heliyon.2021.e06426 [view publication]
    • Product: CoV-SACE2-1
    • Sample Type: Small molecule
  3. Olaleye, Omonike A et al. "Ambroxol Hydrochloride Inhibits the Interaction between Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein’s Receptor Binding Domain and Recombinant Human ACE2." bioRxiv 2020.09.13.295691; doi: https://doi.org/10.1101/2020.09.13.295691 [view publication]
    • Product: CoV-SACE2-1
    • Sample Type: Small molecule
  4. Tsukinoki, Keiichi et al. "Detection of cross-reactive IgA against SARS-CoV-2 spike 1 subunit in saliva." medRxiv 2021.03.29.21253174; doi: https://doi.org/10.1101/2021.03.29.21253174 [view publication]
    • Product: CoV-SACE2-1
    • Sample Type: Purified antibody

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