COVID-19 ELISA

High Sensitivity Assays for IgG, IgM, IgA, and N/S-Proteins

An Enzyme-Linked Immunosorbent Assay (ELISA) is a common biochemical technique that can be used to detect antigens or antibodies, depending on the type of test used. There are several types that have been used in a variety of aspects of COVID19 research, including sandwich-based ELISA and indirect ELISA. The sandwich-based ELISA method is used for the quantitative measurement of soluble proteins in biological fluids. The indirect ELISA method is most suitable for determining total antibody concentrations in a sample.

Current testing for the SARS-CoV-2 virus is limited, and compared to RT-qPCR, ELISA is a less complex procedure that uses more affordable and available equipment. Similarly, antigens and antibodies are considerably more stable than RNA, which reduces the potential of false-negative results. The ability to collect samples from many places in the body (and not restricted to nasal swabs) improves testing accuracy as well.

Sandwich-based ELISA measuring viral antigens could be an alternative to RT-qPCR to detect current infections.

Alternatively, serology measurements rely entirely on a host’s antibody response to the virus rather than detecting the virus itself. Indirect ELISA has been used to 1) diagnose patients who are more than 1 week post symptom onset, 2) determine potential immunity and risk of infection, 3) advance contact tracing, and 4) understand the extent of COVID19 spread and immunity in communities through epidemiological studies that are particularly important for fighting COVID-19 while minimizing economic impact.

Products

Indirect ELISA - In-Vitro Diagnostic Use IVD 35% off through July 31!

Indirect ELISA - Research Use Only (RUO) 35% off through July 31!

Sandwich-based ELISA - Research Use Only (RUO)

FAQs

• Should I use the IgG, IgM, or IgA kit?

  IgG is the most abundant antibody isotype (~70 75%) in the blood. IgG is predominantly responsible for long term immunity after infection.

  IgM accounts for ~10% of antibodies in the blood and is produced first in response to acute infection.

  IgA accounts for another ~10% of blood antibodies and is primarily responsible for protecting mucosal surfaces, which is why it could be especially important in COVID19. It has also been linked to disease severity. Testing for all three antibodies has improved sensitivity over any single antibody testing.

• Can these kits be used for diagnostics?

  The IgG ELISA kits (cat #: IEQ CoVS1RBD IgG and IEQ CoVN IgG) have not been reviewed by the FDA, but may be used for in vitro diagnostics and following FDA policy for the public health emergency. The IgA and IgM ELISAs are for research use only.

• Have these kits been used with real patient samples?

  Yes, they have been tested with samples from patients confirmed to have or not to have COVID 19 via PCR. Please contact us for more details.

• Do you provide testing services?

  Yes, if you do not have access to the laboratory equipment required for these tests, we provide a full testing service. Just send your samples to us