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COVID-19 Spike-ACE2 Binding Assay Kit

The RayBio® COVID-19 Spike-ACE2 binding assay kit is a rapid, simple, and sensitive method to characterize the binding affinity of the SARS-CoV-2 Spike (S) protein and the Angiotensin I Converting Enzyme 2 (ACE2) receptor complex in the presence of potential inhibitors.

$487.00  
CoV-SACE2-1
+
Note:
The binding assay comes in two different options based on which protein is immobilized on the plate and which protein is added in solution (i.e. "query"). It is the researcher's responsibility to select the format for their study.

Antigen Information

Gene Symbols:
  • ACE2
Protein Name & Synonyms:
Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, Angiotensin-converting enzyme homolog, ACEH, Metalloprotease MPROT15, Processed angiotensin-converting enzyme 2, ACE2

Assay Format

Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Plasma
  • Serum
  • Small Molecules
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Application Notes

  • Evaluate inhibitor activity
  • Screen inhibitors of the SARS-CoV-2 Spike-ACE2 complex
  • Characterize antibody function
  • Vaccine development
  • Selection of patient serum for antibody therapy

Storage/Stability

Store kit at 4°C for up to 1 month from the date of shipment. For extended storage, store Items C, D, and F at -20°C. Avoid repeated freeze-thaw cycles.

Great kit at an affordable cost. Easy to use. Have the option to choose Spike or ACE 2 on plate. Overnight incubation yields very high OD at 450nm, but the incubation time could be adjusted to 1-2hours at RT. It would be beneficial if they could provide the concentration of 1X ACE2 protein and also could have included a positive control (a known inhibitor) in the kit.

University of Mississippi
Instructions are easy to follow. Works well with both kit-provided and our own RBD protein. Only suggestion is they should at least disclose the concentration of the query protein.

Scripps Research
The kit works as expected. Easy to use.

National Institutes of Health Clinical Center
I ordered the kit when there was only one option, "Spike RBD on plate + ACE2 query (CoV-SACE2)". At that time, the storage condition was -20°C on arrival, keeping some at -20°C and some at 4°C. I mentioned this for we should take care of the storage of the components of the kit.

UMichigan
We obtained good results using this, so I'll be purchasing more. With that said, the instructions could have been written a bit clearer and the actual starting amount of ACE2 in vial F should be provided (phone operator said it was "unknown"). This makes assigning true values to the standard curve impossible. But as I said, the qualitative use for binding inhibition assays seems to work just fine, just can't be quantitative.

University of Nebraska Medical Center
Great kit. Easy to use. I love how the rows of wells can pop out of the plate so the unused ones can remain in the fridge.

Columbia University
The Spike-ACE2 binding assay is a sensitive and very suitable method for fast screening of potential inhibitors of the S-ACE2 complex formation. Signal intensity at 450 nm recorded for technical replicates results in very similar measurements and the signal-to-noise ratio is very high.

Pharma
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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