Since 2006, RayBiotech has provided Custom Protein Services to produce recombinant proteins based on a customer's specifications. Our comprehensive service program covers every step of production, from gene cloning through protein purification. We can also perform downstream processing like protein desalting, lyophilization, vialing, and labeling.
RayBiotech offers bacterial or mammalian gene expression systems to serve a variety of research requirements.
TruExp™ is a state-of-the-art mammalian cell-based expression system developed by RayBiotech for producing recombinant proteins with native conformations and PTMs. TruExp™ is an excellent option for protein that have a high molecular weight or are difficult to express.
|Serpin B4, Serpin Peptidase Inhibitor||SCCA2||335.7|
|14-3-3 Protein Epsilon||YWHAE||103.1|
|N-terminal of the prohormone BNP||NT-proBNP||99.5|
|Brain natriuretic peptide 32||BNP-32||51.2|
|Receptor Tyrosine-Protein Kinase erb-3||erbB-3||37.5|
|Epidermal Growth Factor Receptor||EGFR||23.5|
|Pepsinogen II, Pepsinogen C||PGC||18.6|
|Lymphocyte Activation Gene 3 Protein||LAG-3||16.9|
|Receptor Tyrosine-Protein Kinase erbB-2||Erb/Her2||16.7|
|Hepatocyte Growth Factor Receptor||HGF R||14.6|
|Golgi Membrane Protein 1||GP73||5.5|
|Characteristic||Bacteria||Yeast||Baculovirus Insect Cells||Mammalian Cells|
|Cell Growth||Rapid (30 min)||Rapid (90 min)||Slow (18-24 hr)||Slow (24 hr)|
|Timeline||3 weeks||3-4 weeks||6-8 weeks||4-5 weeks|
|Post-Translational Modifications (PTMs)|
|Protein Folding||Not reliable||Usually reliable||Very reliable||Very reliable|
|Glycosylation||None||Yes. Not identical to mammalian system. High mannose.||Yes. Not identical to mammalian system. Simple, no sialic acid.||Yes. Complex/Authentic|
This phase evaluates gene expression, protein expression levels, protein secretion, and protein solubility. If the protein does not meet the customer's expectation, the service can be stopped after this phase.