Human RTK Phosphorylation Array G1

$475.00
AAH-PRTK-G1-4

RayBio® G-Series Human Receptor Tyrosine Kinase Phosphorylation Antibody Array 1 Kit. Detects 71 Human RTKs. Suitable for all liquid sample types but intended for use with cell and tissue lysates.

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Antigen Information

Gene Symbol:
  • ABL1
  • AXL
  • BLK
  • BMX
  • BTK
  • CSF1R
  • CSK
  • DDR2
  • EGFR
  • EPHA1
  • EPHA2
  • EPHA3
  • EPHA4
  • EPHA5
  • EPHA6
  • EPHA7
  • EPHA8
  • EPHB1
  • EPHB2
  • EPHB3
  • EPHB4
  • EPHB6
  • ERBB2
  • ERBB3
  • ERBB4
  • FER
  • FGFR1
  • FGFR2
  • FGR
  • FLT4
  • FRK
  • FYN
  • HCK
  • IGF1R
  • INSR
  • ITK
  • JAK1
  • JAK2
  • JAK3
  • KDR
  • KIT
  • LCK
  • LTK
  • LYN
  • MATK
  • MET
  • MUSK
  • NGFR
  • NTRK2
  • PDGFRA
  • PDGFRB
  • PTK2
  • PTK2B
  • RET
  • ROR1
  • ROR2
  • ROS1
  • RYK
  • SLPI
  • SRMS
  • SYK
  • TEC
  • TEK
  • TIE1
  • TNK1
  • TNK2
  • TXK
  • TYK2
  • TYRO3
  • ZAP70

Assay Format

Pathway:
  • PI3K-AKT Signaling
Number of Targets Detected:
71
Species Detected:
  • Human
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants
  • Cell Lysates
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
ABL1
  • Gene Symbol: ABL1
  • Gene ID: 25
  • Accession #: P00519
ACK
ALK-1
Axl
Blk
BMX
Btk
Csk
Dtk
EGFR
EphA1
EphA2
EphA3
EphA4
EphA5
EphA6
EphA7
EphA8
EphB1
EphB2
EphB3
EphB4
EphB6
ErbB2
ErbB3
ErbB4
FAK
FER
FGFR1
FGFR2
FGFR2 alpha isoform
  • Gene Symbol: FGFR2
  • Gene ID: 2263
  • Accession #: P21802
Fgr
FRK
Fyn
Hck
HGFR
IGF-1 R
Insulin R
(CD220)
Itk
JAK1
JAK2
JAK3
LCK
LTK
Lyn
MATK
M-CSF R
MUSK
NGFR
(TNFRSF16)
PDGFRA
PDGFRB
PYK2
RET
ROR1
ROR2
ROS
RYK
SCF R
(CD117/c-kit)
SRMS
SYK
Tec
Tie-1
Tie-2
TNK1
TRKB
TXK
Tyk2
TYRO10
(DDR2/TKT)
VEGFR2
VEGFR3
ZAP70

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human RTK Phosphorylation Antibody Array G1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Protease Inhibitor Cocktail
  • Phosphatase Inhibitor Cocktail II
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Highly informative setup that answers many initial questions and provides insights on future directions. The protocol is easy to follow and not very time consuming. The final results came out clear with high degree of reproducibility. Very low background and great signal to noise ratio. On the less positive side I would mention high cost of the kit. But given the fact the kit provides with great amount of data the one might consider the price factor as worthwhile.
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Analysis Tool Bundle Discount

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1 x $475.00
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1 x $115.00 $34.50

70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Total list price $590.00
Price for all $509.50

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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