Name: Glucose-6-Phosphate Dehydrogenase, Human Recombinant
SKU: 228-20555-2
Availability: InStock

G6PD, G6PD1, Glucose-6-phosphate 1-dehydrogenase.

MGSSHHHHHH SSGLVPRGSH MAEQVALSRT QVCGILREEL FQGDAFHQSD THIFIIMGAS GDLAKKKIYP TIWWLFRDGL LPENTFIVGY ARSRLTVADI RKQSEPFFKA TPEEKLKLED FFARNSYVAG QYDDAASYQR LNSHMNALHL GSQANRLFYL ALPPTVYEAV TKNIHESCMS QIGWNRIIVE KPFGRDLQSS DRLSNHISSL FREDQIYRID HYLGKEMVQN LMVLRFANRI FGPIWNRDNI ACVILTFKEP FGTEGRGGYF DEFGIIRDVM QNHLLQMLCL VAMEKPASTN SDDVRDEKVK VLKCISEVQA NNVVLGQYVG NPDGEGEATK GYLDDPTVPR GSTTATFAAV VLYVENERWD GVPFILRCGK ALNERKAEVR LQFHDVAGDI FHQQCKRNEL VIRVQPNEAV YTKMMTKKPG MFFNPEESEL DLTYGNRYKN VKLPDAYERL ILDVFCGSQM HFVRSDELRE AWRIFTPLLH QIELEKPKPI PYIYGSRGPT EADELMKRVG FQYEGTYKWV NPHKL

Sterile Filtered colorless solution.

The G6PD solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1mM PMSF, 2mM EDTA, 2mM DTT, 200mM NaCl and 20% glycerol.

Specific activity is > 45 units/ml by measuring the increase of NADPH at 340 nm resulting from the reduction of β NADP. One unit oxidizes 1.0 µmole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of β-NADP at pH 7.4 at 25°C.

Storage/Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Description

G6PD Human Recombinant produced in Hi-5 cells is a single polypeptide chain containing 535 amino acids (1-515) and having a molecular mass of 61.4kDa.G6PD is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

G6PD, G6PD1, Glucose-6-phosphate 1-dehydrogenase.

Sterile Filtered colorless solution.

The G6PD solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1mM PMSF, 2mM EDTA, 2mM DTT, 200mM NaCl and 20% glycerol.

Storage/Stability

Description

Catalog #:228-20555-2

Size	20 µg, 1000 µg
Estimated Lead Time	2-3 weeks
Species	Human
Accession Number	P11413
Gene Id	2539
Protein Name / Synonyms	G6PD, G6PD1, Glucose-6-phosphate 1-dehydrogenase.
Expressed Region	MGSSHHHHHH SSGLVPRGSH MAEQVALSRT QVCGILREEL FQGDAFHQSD THIFIIMGAS GDLAKKKIYP TIWWLFRDGL LPENTFIVGY ARSRLTVADI RKQSEPFFKA TPEEKLKLED FFARNSYVAG QYDDAASYQR LNSHMNALHL GSQANRLFYL ALPPTVYEAV TKNIHESCMS QIGWNRIIVE KPFGRDLQSS DRLSNHISSL FREDQIYRID HYLGKEMVQN LMVLRFANRI FGPIWNRDNI ACVILTFKEP FGTEGRGGYF DEFGIIRDVM QNHLLQMLCL VAMEKPASTN SDDVRDEKVK VLKCISEVQA NNVVLGQYVG NPDGEGEATK GYLDDPTVPR GSTTATFAAV VLYVENERWD GVPFILRCGK ALNERKAEVR LQFHDVAGDI FHQQCKRNEL VIRVQPNEAV YTKMMTKKPG MFFNPEESEL DLTYGNRYKN VKLPDAYERL ILDVFCGSQM HFVRSDELRE AWRIFTPLLH QIELEKPKPI PYIYGSRGPT EADELMKRVG FQYEGTYKWV NPHKL
Purity	> 95%
Purity Determined By	SDS-PAGE
Format	Sterile Filtered colorless solution.
Formulation	The G6PD solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1mM PMSF, 2mM EDTA, 2mM DTT, 200mM NaCl and 20% glycerol.
Activity	Specific activity is > 45 units/ml by measuring the increase of NADPH at 340 nm resulting from the reduction of β NADP. One unit oxidizes 1.0 µmole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of β-NADP at pH 7.4 at 25°C.
Research Area	Energy Metabolism, Glycolysis & Lipid Metabolism
Source	Hi-5 cells
Shipping Type	Blue ice
Storage	-20°C

Size	20 µg, 1000 µg
Estimated Lead Time	2-3 weeks
Species	Human
Accession Number	P11413
Gene Id	2539
Protein Name / Synonyms	G6PD, G6PD1, Glucose-6-phosphate 1-dehydrogenase.
Expressed Region	MGSSHHHHHH SSGLVPRGSH MAEQVALSRT QVCGILREEL FQGDAFHQSD THIFIIMGAS GDLAKKKIYP TIWWLFRDGL LPENTFIVGY ARSRLTVADI RKQSEPFFKA TPEEKLKLED FFARNSYVAG QYDDAASYQR LNSHMNALHL GSQANRLFYL ALPPTVYEAV TKNIHESCMS QIGWNRIIVE KPFGRDLQSS DRLSNHISSL FREDQIYRID HYLGKEMVQN LMVLRFANRI FGPIWNRDNI ACVILTFKEP FGTEGRGGYF DEFGIIRDVM QNHLLQMLCL VAMEKPASTN SDDVRDEKVK VLKCISEVQA NNVVLGQYVG NPDGEGEATK GYLDDPTVPR GSTTATFAAV VLYVENERWD GVPFILRCGK ALNERKAEVR LQFHDVAGDI FHQQCKRNEL VIRVQPNEAV YTKMMTKKPG MFFNPEESEL DLTYGNRYKN VKLPDAYERL ILDVFCGSQM HFVRSDELRE AWRIFTPLLH QIELEKPKPI PYIYGSRGPT EADELMKRVG FQYEGTYKWV NPHKL
Purity	> 95%
Purity Determined By	SDS-PAGE
Format	Sterile Filtered colorless solution.
Formulation	The G6PD solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1mM PMSF, 2mM EDTA, 2mM DTT, 200mM NaCl and 20% glycerol.
Activity	Specific activity is > 45 units/ml by measuring the increase of NADPH at 340 nm resulting from the reduction of β NADP. One unit oxidizes 1.0 µmole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of β-NADP at pH 7.4 at 25°C.
Research Area	Energy Metabolism, Glycolysis & Lipid Metabolism
Source	Hi-5 cells
Shipping Type	Blue ice
Storage	-20°C

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Glucose-6-Phosphate Dehydrogenase, Human Recombinant

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