Human IL-1 R4 ELISA

RayBio® Human IL-1 R4 (ST2) ELISA Kit for cell culture supernatants, plasma, and serum samples.

$402.00  
ELH-IL1R4-1
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Antigen Information

Accession Number:
  • Q01638
Gene ID:
  • 9173
Gene Symbols:
  • DER4
  • IL1RL1
  • ST2
  • T1
Protein Name & Synonyms:
Interleukin-1 receptor-like 1 (Protein ST2)
Target Species:
  • Human

Assay Format

Detection Range:
2 pg/ml - 1200 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
2 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Ates, Ihsan, et al. "Elevated circulating sST2 associated with subclinical atherosclerosis in newly diagnosed primary hypertension."?Hypertension Research?39.7 (2016): 513-518.
    Species: Human
    Sample type: Serum
  2. Zhang Y, Zhang C, Zhuang R, Ma Y, ZhangY, Yi J, et al. (2015) IL-33/ST2 Correlates withSeverity of Haemorrhagic Fever with RenalSyndrome and Regulates the Inflammatory Responsein Hantaan Virus-Infected Endothelial Cells. PLoSNegl Trop Dis 9(2): e0003514. doi:10.1371/journal.pntd.0003514
    Species: Human
    Sample type: Plasma
  3. Vocca L., Di Sano C., Uasuf CG., et al. IL-33/ST2 axis controls Th2/IL-31 and Th17 immune response in allergic airway diseases. Immunobiology. 2015 Aug;220(8):954-63. doi: 10.1016/j.imbio.2015.02.005.
    Species: Human
    Sample type: Plasma
  4. Alkhiary W, Abdalaal M, El-Sabbagh A (2015) Serum Concentrations of Interleukin-33 and its Soluble Receptor sST2 in Patients with Persistent Atrial Fibrillation. J Mol Biomark Diagn 6:235. doi:10.4172/2155-9929.1000235
    Species: Human
    Sample type: Serum
  5. Lin Y., et al. A panel of four cytokines predicts the prognosis of patients with malignant gliomas. J Neurooncol (2013) 114:199-208, DOI 10.1007/s11060-013-1171-x
    Species: Human
    Sample type: Plasma
  6. Willems S, Sels J-W, Flier S, et al. Temporal changes of soluble ST2 after cardiovascular interventions. Eur J Clin Invest. 2013 Feb;43(2):113-120.
    Species: Human
    Sample type: Plasma
  7. Willems, Sanne, et al. "Soluble ST2 levels are not associated with secondary cardiovascular events and vulnerable plaque phenotype in patients with carotid artery stenosis."?Atherosclerosis?231.1 (2013): 48-53.
    Species: Human
    Sample type: Plasma

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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